常规染色体畸变分析法对AT细胞高辐射敏感性的研究  

Study on High Radiosensitivity of AT Cells by Conventional Chromosome Aberration Analysis Method

作　　者：罗加林[1] 曹建平[1] 朱巍[1] 王明明[1] 周献锋[1] 朱财英[1] 郑斯英[1] 樊赛军[1] 

机构地区：[1]苏州大学放射医学与公共卫生学院放射生物学教研室,江苏苏州215006

出　　处：《苏州大学学报（医学版）》2004年第5期589-591,共3页Suzhou University Journal of Medical Science

基　　金：国家自然科学基金项目(项目编号:30170288)江苏省2004年度研究生创新计划项目(Xm04-67)苏州大学医学发展基金项目

摘　　要：目的 研究毛细血管扩张性共济失调症(ataxia-telangiectasia,AT)患者皮肤的成纤维细胞系AT5BIVA(AT细胞)的高辐射敏感性。方法 以源于正常人皮肤的成纤维细胞系GM0639(GM细胞)为对照,用常规染色体畸变分析法,在AT细胞和GM细咆经60COγ射线0、1、2、3、4 Gy照射后,观察比较AT细胞和GM细胞之间染色体畸变率(CAF)的差异,并分别进行曲线拟合。结果 在0、1、2、3、4 Gy剂量下,AT细胞染色体畸变率明显高于GM细胞(P<0.05);AT和GM细胞染色体畸变率与剂量成正相关,均可拟合成剂量效应直线方程Y=a+bX,且AT细胞剂量效应直线回归方程斜率明显大于GM细胞(P<0.05)。结论 AT患者AT细胞的辐射敏感性显著高于GM细胞,具有高辐射敏感性。Objective To study the high radiosensitivity of fibroblast cells (AT5BIVA cells) from the skin of the patients of ataxia-telangiectasia (AT). Methods Using conventional chromosome aberraton analysis method, chromosome aberration frequence( CAF) of AT cells and GM cells exposed to 60CO γ-rays was observed at doses of 0, 1, 2, 3 and 4 Gg, compared with that of GM 0639(GM cells) from the skin of the normal subject. The difference of CAF between AT cells and GM cells was analyzed, and dose-response curves were established. Results After exposed to 0, 1,2,3 and 4 Gy 60 CO γ-rays, theradiation-induced level of CAF was significantly higher in AT cells compared with GM cells (P<0.05) . In the two cell types, CAF has a positive correlation with dose, and their linear regression equation is Y = a + bX. The slope of the CAF linear regression equation of AT cells is larger than that of GM cells (P<0.05). Conclusion The radiosensitivity of AT cells is significantly higher than that of GM cells. AT cells is extremely sensitive to radiation.

关 键 词：毛细血管扩张性共济失调症 高辐射敏感性 电离辐射 染色体畸变 

分 类 号：R543.7[医药卫生—心血管疾病]