^(60)Coγ射线照射对AT细胞周期进程的影响  

Effects of ^(60)Coγ-Rays Irradition on AT Cell Cycle Progression

作　　者：王明明[1] 周献峰[1] 朱巍[1] 罗加林[1] 曹建平[1] 郑斯英[1] 赵经涌[1] 

机构地区：[1]苏州大学放射医学与公共卫生学院放射生物学教研室,江苏苏州215007

出　　处：《苏州大学学报（医学版）》2004年第5期592-594,共3页Suzhou University Journal of Medical Science

基　　金：国家自然科学基金资助课题(编号30170288)

摘　　要：目的 研究AT细胞辐射高敏感性与细胞周期进程间的联系。方法 用细胞流式术检测2 Gy60Coγ射线照射后不同时间及不同剂量照射后GM0639和AT5BIVA两种细胞的细胞周期分布状况。结果 GM0639细胞受2 Gy60Coγ射线照射后,从照射后即刻至第24小时,G2／M细胞比例逐步增大,出现了G2阻滞,0~6 Gy60Coγ射线照射24 h后,G2／M细胞比例随剂量增大而增大;AT5BIVA受2 Gy60Coγ射线照射后不同时间及0-6 Gy60Coγ射线照射24 h后,G2／M细胞比例减小,不发生G2阻滞。结论 ATM基因突变所导致的细胞周期关卡丧失及DNA修复能力降低是AT细胞辐射高敏感性的重要原因。Objective To explore the relationship between cell cycle progression and high radiosensitivity of AT5BIVA cells. Methods Using flow cytometry to determine cell cycle distribution of AT5BIVA and GM0639 cells from 0-24 h after exposure to 2 Gy 60Co 7- rays and at the 24th h after exposure to 0-6 Gy 60Coγ- rays. Results After GM0639 cells were exposed to 2 Gy 60Coγ-rays, the percentage of G2/M cell increased with prolonged time. The percentage of G2/M cell augmented with irradiated dose at the 24th h in GM0639 cells exposed to 0-6 Gy 60Coγ- rays. The percentage of G2/M cells decreased with prolonged time and irradiated dose from 0 ~ 24 h after AT5BIVA cells were exposed to 2 Gy 60 Coγ- rays and at the 24th h after AT5BIVA cells were exposed to 0-6 Gy 60 Coγ-rays. Conclusion The loss of cell cycle check points and the disfunction of DNA repair caused by ATM gene mutation may be responsible for high radiosensitivity of AT5BIVA cells.

关 键 词：ATM基因 GM0639细胞 AT5BIVA细胞 细胞周期 

分 类 号：R811.5[医药卫生—放射医学]