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作 者:邓欢[1] 陈俅[1] 苏浩[1] 张东升[2] 马志强[1]
机构地区:[1]辽宁省海洋水产研究所辽宁省应用海洋生物开放性实验室,辽宁大连116023 [2]大连水产学院生命科学与技术学院,辽宁大连116023
出 处:《大连水产学院学报》2004年第4期258-263,共6页Journal of Dalian Fisheries University
基 金:辽宁省水产厅重点科研项目资助(9810);大连市科技攻关项目资助(962020606)
摘 要:从辽宁兴城育苗场和辽宁省海洋水产研究所育苗室患面盘解体的海湾扇贝ArgopectenirradiansLamarck幼体中分离出6株菌,经感染试验表明,其中3株菌B01、Y01和Y02为病原菌。根据菌体形态及生理、生化测试,B01鉴定为需钠弧菌Vibrionatriegen,Y01和Y02具有相同的形态、生理、生化特征,鉴定为弧菌属Vibrio。需钠弧菌对庆大霉素、痢特灵、菌必治、诺氟沙星、氯霉素、环丙沙星、多粘菌素B敏感;Y01和Y02菌对诺氟沙星、菌必治、氯霉素、复方新诺明、环丙沙星、磺胺异唑敏感。对海湾扇贝育苗水体中细菌总数和弧菌数量的监测结果表明,幼体发生病变之前的水体中,细菌和弧菌的密度高于正常育苗水体的。文中还对病原菌的传播途径等问题进行了讨论。Six bacterial strains were isolated from the larval bay scallop Argopecten irradians when epizootic occurred in the hatcheries near Bohai sea and Yellow sea. The pathogenicity assays showed that the strains B01, Y01, Y02 could cause the larval bacillary necrosis, but the other three strains could not. The strain B01 was identified as Vibrio natriegen morphologically, physiologically and biochemically. Strains Y01, Y02 possessed the same characteristics as B01 and thus belonged to Vibrio genus. The drug susceptibility tests indicated that Vibrio natriegen had sensibility to gentamycin, furazolidone, norfloxacin ceftriaxone, chloramphennicol, ciprofloxacin, and polymyxin B; strains Y01, Y02 were sensible to ciprofloxacin, gentamcecin, norfloxacin, chloramphenicol, ceftriaxone, SMZ/TMP, and SIZ. High bacterial density was found in the water prior to the larval epizootics occurred. The routes of pathogens transmission were discussed.
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