体外人巨细胞病毒感染与核转录因子AP-1活化及原癌基因c-jun表达的研究  

Activation of Nuclear Transcription Factor AP-1 and the Expression of Proto-oncogene C-jun during Human Cytomegalovirus Infection

作　　者：程敏[1] 闻良珍[1] 凌霞珍[1] 赵捷[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,武汉430030

出　　处：《华中科技大学学报（医学版）》2004年第3期335-338,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家"九五"科技攻关项目 (No .96 90 4 0 6 0 8)

摘　　要：目的　研究人胚肺成纤维 (HEL)细胞感染人巨细胞病毒 (HCMV)后 ,转录激活蛋白 1(AP 1)活化和原癌基因c junmRNA表达的时序性变化 ,探讨其在HCMV感染中的作用。方法　采用原位杂交法检测c junmRNA的表达 ,凝胶电泳迁移率改变试验 (EMSA)检测AP 1活性。结果　HEL细胞感染HCMV后 15min ,c junmRNA即有阳性表达 ,30～ 6 0min达高峰 (P <0 0 1) ,以后逐渐下降 ,正常对照组HEL细胞无c junmRNA表达。HCMV感染后AP 1亦迅速被活化 ,至 2h达高峰 (P <0 0 1) ,其后有所下降 ,维持于一定水平。结论　HEL细胞感染HCMV后 ,c junmRNA表达迅速增加 ,AP 1活化。提示HCMV可能通过刺激原癌基因过度表达而参与细胞的信号传导 ,以干扰细胞增殖和分化的调控 ;AP 1活化为早期病毒基因有效表达所必需 ,并可启动一系列前炎性细胞因子的基因转录与蛋白合成。Objective To investigate the scheduling change of the activation of AP-1 and the expression of proto-oncogene c-jun mRNA in HCMV-infected HEL cells so as to explore the role of c-jun and AP-1 in HCMV infection. Methods The expression of c-jun mRNA was detected by in situ hybridization (ISH) and activity of AP-1 was measured by electrophoretic mobility shift assay (EMSA).Results The expression of c-jun mRNA was detected in the infected HEL cells at 15 min post infection, reached the peak at 30—60 min (P<0.01), then slowed down, while there was no expression of c-jun mRNA in the uninfected HEL cells. AP-1 was also activated rapidly post HCMV infection and reached the highest level at 2 h (P<0.01), then declined to some extent and maintained at a certain level. Conclusion Post HCMV infection, the expression of c-jun mRNA was increased and AP-1 was activated. It suggested that HCMV might participate in the signal transduction via stimulating the overexpression of proto-oncogene and the activation of AP-1 may be necessary for the early efficient expression of HCMV gene.

关 键 词：AP-1 原癌基因C-JUN HCMV感染 mRNA表达 人巨细胞病毒感染 体外 核转录因子 HEL细胞 转录激活蛋白 凝胶电泳 

分 类 号：R735[医药卫生—肿瘤] R737[医药卫生—临床医学]