淋巴组织免疫组织化学染色及质量控制  

Immunohistochemistry Staining of Lymphoid Tissues and Quality Control

作　　者：袁永辉[1] 李建莎[1] 张韵风[1] 陈多恩[1] 

机构地区：[1]华中科技大学同济医学院基础医学院病理学系,武汉430030

出　　处：《华中科技大学学报（医学版）》2004年第3期382-384,共3页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的　建立淋巴组织的制片与免疫组化的标准化实验方法 ,达到临床病理检验质控要求。方法　详细介绍了淋巴组织的取材、固定、脱水、透明、石蜡包埋、切片、免疫组化和TUNEL染色方法及其质量控制环节。结果　用此法制备的免疫组化切片具有阳性结果呈颗粒状的棕黄色 ,定位准确 ,对比明显 ,背景浅 ,组织结构与细胞轮廓完整、清晰 ,适合显微照相以及染色结果稳定可靠 ,特异性强、敏感性高。结论　该方法可作为淋巴组织的制片与免疫组化的标准化实验方法应用。Objective To establish a standard experimental method for making paraffin-embedded sections and staining of immunohistochemistry in lymphoid tissues to meet the quality control demand in clinicopathologic tests. Methods The procedures for sampling, formalin-fixation, dehydration, clearing, paraffin embedding, cutting into sections and staining of immunohistochemistry and terminal-deoxynucleotidyl transferase mediated x-dUTP nick end labeling (TUNEL) of lymphoid tissues were described in detail. Results The sections by this method could show special yellow granule in positive cells; They revealed the more accurate location, markedly characteristic contrast, weaker background with good visibility, more detail and clear structure of tissues and cells, and were suitable for microphotography. Also staining results by this method were stable and reliable with strong specificity and high sensitivity. Conclusion This method can be applied as a standard experimental method to make paraffin-embedded sections and staining of immunohistochemistry and TUNEL in lymphoid tissues.

关 键 词：淋巴组织 免疫组化 免疫组织化学染色 制片 TUNEL 阳性结果 质控 颗粒状 制备 透明 

分 类 号：R735[医药卫生—肿瘤] R733[医药卫生—临床医学]