Effect of polydatin on phospholipase A_2 in lung tissues in rats with endotoxic shock  

作　　者：舒仕瑜 王兴勇 凌智瑜 卢仲毅 

机构地区：[1]Department of Anesthesia,Children's Hospital,Chongqing University of Medical Sciences [2]Department of Emergency,Children's Hospital,Chongqing University of Medical Sciences [3]Department of Cardiology,Second Affiliated Hospital,Chongqing University of Medical Sciences

出　　处：《Chinese Journal of Traumatology》2004年第4期239-243,共5页中华创伤杂志（英文版）

基　　金：This projectwassupportedbytheChineseMedicalAdministrationBureauofChongqingCity (No .2 0 0 2 4 2 2 1)

摘　　要：Objective: To study the effect of polydatin on p hospholipase A2 in lung tissues in rats with endotoxic shock. Methods: Thirty-two healthy male Wistar rats were employed in this study. A total of 8 rats received normal saline intravenously （control grou p）,8 rats received 10 mg/kg of endotoxin （endotoxic shock group）,8 rats re ceived 1 mg/kg of polydatin after endotoxin injection （polydatin treatment g roup）,and 8 rats received 1 mg/kg of polydatin （polydatin prevention group） 30 minutes before endotoxin injection. Mean arterial pressure was measured once hal f an hour. Lung tissues were collected 6 hours later. Phospholipase A2 activit y was measured with acid titration. The gene expression of secretory phospholipa se A2 type IIA was detected with reverse transcription polymerase chain reacti on. Meanwhile,the histological changes of the lungs among four groups were comp ared through microscopic examination.Results: Phospholipase A2 activity and the gene expression of secretory phospholipase A2 type IIA increased after endotoxin injection,but polydatin could inhibit these effects of endotoxin. Obvious morphological eviden ce could be found in the lung pathological sections and the protective effect of polydatin was most significant in the polydatin prevention group.Conclusions: Polydatin has prophylactic and therapeutic effects （the former is more distinct than the latter） on acutely injured lungs in rats with endotoxic shock and which suggests that polydatin may be a phospholipase A 2 inhibitor.

关 键 词：Shock septic Phospholipases A POLYDATIN 

分 类 号：R563[医药卫生—呼吸系统]