“Co-culture Engineering”for Enhanced Phytoremediation of Metal Contaminated Soils  

作　　者：NICai-Ying SHIJi-Yan LUOYong-Ming CHENYing-Xu 

机构地区：[1]DepartmentofEnvironmentalEngineering,SchoolofEnvironmentandResourceSciences,ZhejiangUniversity,Hangzhou310029（China）. [2]SoilandEnvironmentBioremediationResearchCentre,InstituteofSoilScience,ChineseAcademyofSciences,Nanjing210008（China）

出　　处：《Pedosphere》2004年第4期475-482,共8页土壤圈（英文版）

基　　金：Project supported by the National Natural Science Foundation of China (Nos. 40271060 and 41025005) ; the National Key Basic Research Support Foundation (NKBRSF) of China (No. 2002CB410809/10).

摘　　要：A co-culture of two plant materials, Astragalus sinicus L., a leguminous plant with concomitant nodules, and Elsholtzia splendens Naki-a Cu accumulator, along with treatments of a chelating agent (EDTA), root excretions (citric acid), and a control with E. splendens only were used to compare the mobility of heavy metals in chelating agents with a co-culture and to determine the potential for co-culture phytoremediation in heavy metal contaminated soils. The root uptake for Cu, Zn, and Pb in all treatments was significantly greater (P ＜ 0.05) than that of the control treatment. However with translocation in the shoots, only Cu, Zn, and Pb in plants grown with the EDTA treatment and Zn in plants cocropped with the A. sinicus treatment increased significantly (P ＜ 0.05). In addition, when a co-culture in soils with heavy and moderate contamination was compared, for roots in moderately contaminated soils only Zn concentration was significantly less (P ＜ 0.05) than that of heavily contaminated soils, however, Cu, Zn, and Pb concentrations of shoots were all significantly lower (P ＜ 0.05). Overall, this 'co-culture engineering' could be as effective as or even more effective than chelating agents, thereby preventing plant metal toxicity and metal leaching in soils as was usually observed in chelate-enhanced phytoremediation.A co-culture of two plant materials, Astragalus sinicus L., a leguminousplant with concomitant nodules, and Elsholtzia splendens Naki—a Cu accumulator, along withtreatments of a chelating agent (EDTA), root excretions (citric acid), and a control with E.splendens only were used to compare the mobility of heavy metals in chelating agents with aco-culture and to determine the potential for co-culture phytoremediation in heavy metalcontaminated soils. The root uptake for Cu, Zn, and Pb in all treatments was significantly greater(P < 0.05) than that of the control treatment. However with translocation in the shoots, only Cu,Zn, and Pb in plants grown with the EDTA treatment and Zn in plants co-cropped with the A. sinicustreatment increased significantly (P < 0.05). In addition, when a co-culture in soils with heavy andmoderate contamination was compared, for roots in moderately contaminated soils only Znconcentration was significantly less (P < 0.05) than that of heavily contaminated soils,however, Cu,Zn, and Pb concentrations of shoots were all significantly lower (P < 0.05). Overall, this'co-culture engineering' could be as effective as or even more effective than chelating agents,thereby preventing plant metal toxicity and metal leaching in soils as was usually observed inchelate-enhanced phytoremediation.

关 键 词：Astragalus sinicus chelating agents CO-CULTURE Elsholtzia splendens PHYTOREMEDIATION 

分 类 号：X703[环境科学与工程—环境工程]