肝硬化大鼠骨代谢及其调控激素的改变  

Study on the Changes of Bone Metabolism and Its Modulating Hormones in CCl_4-induced Liver Cirrhosis Rats

作　　者：俞华芳[1] 朱金水[2] 余小虎[2] 

机构地区：[1]复旦大学附属金山医院消化科,上海200540 [2]上海第六人民医院消化内科,上海200231

出　　处：《同济大学学报（医学版）》2004年第5期381-384,共4页Journal of Tongji University(Medical Science)

摘　　要：目的　研究肝硬化大鼠骨代谢及其调控激素的改变。方法　 2 0只大鼠分为 2组 ,一组以四氯化碳肌注建立肝硬化大鼠模型 ,另一组为正常对照组。分别测定 2组的肝功能 [丙氨酸氨基转移酶 (aspartameaminotransferase ,ALT)、总胆红素 (totalbilirubin ,TB)、白蛋白 /球蛋白 (albumin/ globeprdein ,A/G) ],股骨和腰椎骨密度 (BMD) ,骨代谢指标 [血清钙 ,2 5羟基维生素D3 ,甲状旁腺激素 (parathyroid ,PTH) ,尿吡啶酚 /肌酐 (pyridinoline/creatinine ,Pyd/Cr) ]。结果　肝硬化大鼠与正常对照组相比 ,股骨和腰椎骨密度显著下降 [(0 .1737± 0 .0 10 0vs 0 .192 0± 0 .0 104 )g/cm2 ,P <0 .0 1;(0 .175 9± 0 .0 0 75vs 0 .16 4 2± 0 .0 10 8)g/cm2 ,P <0 .0 1],血清 2 5 (OH)VitD3 显著下降 [(2 0 .1± 3.2 7vs 15 .6± 2 .4 7) μg/L ,P <0 .0 1],尿Pyd/Cr显著升高 [(4 .8± 0 .8vs 75 .8± 6 0 .7)nmol/mmolCr,P <0 .0 1],A/G显著下降 [(0 .98± 0 .1vs 0 .79± 0 .1) ,P <0 .0 1],而PTH水平改变无统计学意义。相关分析显示 ,血清A/G与股骨和腰椎骨密度呈正相关 (r =0 .70 2 ,P <0 .0 1;r =0 .6 5 9,P <0 .0 1) ;2 5 (OH)VitD3 与椎骨骨密度、股骨骨密度呈显著正相关 (r =0 .75 1,P <0 .0 1) ,(r =0 .Objective To investigate the bone metabolism of CCl4-induced liver cirrhosis rats.Methods Twenty SD rats were divided into 2 groups:10 CCl4-induced liver cirrhosis rats and 10 controls.The liver function parameters such as aspartate aminotransferase(AST),alanine aminotransferase(ALT),gamma-glutamyl ransferase (γ-GT),serum total protein(TP),total bilirubin(TB) were measured by auto biochemistry analysis instruments.Bone mineral density (BMD) of lumbar vertebra and femoral bone were measured by double-energy X ray BMD instrument and bone metabolic parameters including parathyroid hormone (PTH),urinary pyridinoline/creatinine(Pyd/Cr) were determined in the auto chemiluminescince assay instrument.Serum 25(OH)VitD 3 was assayed by ELISA.Results Compared with the controls,BMD in lumbar and femoral bone significantly decreased[(0.1737±0.01 vs 0.1920±0.01)g/cm2,P<0.01;(0.1759±0.007 vs 0.1642±0.010)g/cm2,P<0.01].Serum 25(OH)Vit D 3 of cirrhosis rats was lower than that of the controls[( 15.6 ±2.47 vs 20.1±3.27)μg/L,P<0.01],while urinary Pyd/Cr of cirrhosis rats was significantly higher than that of the control.Conclusion There is osteoporosis in CCl4-induced liver cirrhosis rats. Main changes of bone metabolism in liver cirrhosis are the declination of the level of serum 25(OH)Vit D 3 and the elevation of urinary Pyd/Cr.The results suggest the declined serum 25(OH)VitD 3 in cirrhosis rats may cause osteoporosis by accelerating bone resorption and bone dissolution.

关 键 词：肝硬化 骨代谢 肝功能 骨密度 大鼠 

分 类 号：R575[医药卫生—消化系统]