环氧合酶-2和血管内皮生长因子-C与胃癌淋巴管转移  

The role of cyclooxygenase-2 and vascular endothelial growth factor-C in lymphatic metastasis of gastric cancer

作　　者：刘晋[1] 于皆平[1] 王晓玲[1] 周晓东[1] 于红刚[1] 

机构地区：[1]武汉大学人民医院消化科,武汉430060

出　　处：《中华内科杂志》2004年第11期841-844,共4页Chinese Journal of Internal Medicine

基　　金：国家自然科学基金资助项目(3 0 3 0 0 15 4)

摘　　要：目的　研究环氧合酶 2 (COX 2 )和血管内皮生长因子 (VEGF) C在胃癌组织中的表达及相关性 ,探讨二者在胃癌淋巴管生成和转移中的作用。方法　采用免疫组化方法和逆转录 PCR技术 ,分别检测了 6 4例胃癌石蜡组织中COX 2和VEGF C的表达及其中 2 2例胃癌新鲜组织中二者mRNA的表达。结果　 2 2例胃癌新鲜组织中COX 2和VEGF CmRNA表达阳性率分别为 82 %和73% ,其表达均高于相应的癌旁正常组织 (P <0 0 0 1) ,与 6 4例胃癌石蜡标本COX 2和VEGF C的表达结果较一致。且COX 2和VEGF C表达之间存在明显关联性 (P <0 0 5 )。二者在癌组织中的高表达与肿瘤浸润深度、淋巴结转移等密切相关 (P <0 0 5 )。结论　胃癌组织中有COX 2和VEGF C的高表达 ,而COX 2可能参与VEGF C淋巴管生成通路 。Objective It has been known that cyclooxygenase-2(COX-2) and vascular endothelial growth factor(VEGF)-C are closely related with the development of tumors. To investigate the expression of COX-2 and VEGF-C in gastric cancer and the correlation between them . Methods Sixty-four paraffin-embedded gastric cancer samples and 22 flesh gastric cancer samples were tested with using immunohistochemistry method and the reverse transcription -polymerase chain reaction technology respectively.Results The mean expressive density of COX-2 and VEGF-C mRNA in gastric cancer were both significantly higher than those in non-cancerous gastric mucosa (1.363±0.351 vs 0.763±0.304, 0.972±0.331 vs 0.314±0.215, P <0.001). The positive rates of COX-2 and VEGF-C in 64 gastric cancer samples were 72% and 64% respectively. Their expression in the groups of lymph-node metastasis were higher than that of the non-lymph-node metastasis groups respectively( P <0.05). Moreover, there was a close correlation between COX-2 and VEGF-C expression levels( P <0.05).Conclusions There are high COX-2 and VEGF-C expression in gastric cancer. Gastric tumor tissues that produce VEGF-C may have a higher lymphatic invasion and metastatic potential. COX-2 may participate in VEGF-C lymphangiogenic pathway and the high expression of them may play an important role in the lymphatic proliferation and spread in gastric cancer.

关 键 词：COX-2 胃癌 VEGF-C 高表达 淋巴管生成 环氧合酶-2 血管内皮生长因子-C 新鲜组织 石蜡标本 RNA 

分 类 号：R735.2[医药卫生—肿瘤]