海藻氨酸致癫状态大鼠海马区神经元损伤与Mg^(2+)作用的研究  

Study on neuronal necrosis in hippocampus of kainic acid induced status epilepticus in rats and effect of Mg ^(2+)

作　　者：刘学伍[1] 迟令懿[1] 陈善红 吴伟[1] 孙建英[1] 王慧煜[1] 迟兆富[1] 

机构地区：[1]山东大学齐鲁医院神经内科,济南250012 [2]山东省沂源县人民医院内科

出　　处：《临床神经病学杂志》2004年第5期342-344,共3页Journal of Clinical Neurology

基　　金：山东省自然科学基金资助 (Y2 0 0 1C1 0 )

摘　　要：目的　观察海藻氨酸 (KA)诱导的癫疒间 状态 (SE)大鼠海马神经元的形态学改变和Mg2 + 的神经保护作用。方法　选用成年雄性Wistar大鼠 75只 ,随机分为KA组、Mg2 + 组和生理盐水对照组。用KA诱导大鼠SE 3h ,Mg2 + 组大鼠在注射KA前腹腔内注射硫酸镁 10 0mg/kg ,在癫疒间 发作终止后 72h将动物处死 ,分别用光镜和电镜观察海马神经元形态学改变。结果　KA组大鼠注射KA后 (16 .1± 4 .7)min出现癫疒间 发作 ,Mg2 + 组大鼠为 (2 5 .4± 6 .2 )min ,两组比较差异有显著性 (P <0 0 5 )。KA组和Mg2 + 组大鼠在海马区均出现了嗜酸性神经元 ,Mg2 + 组大鼠神经元损伤程度明显低于KA组。结论　KA诱导的SE可导致海马神经元坏死 ,而Mg2 + 作为兴奋性氨基酸拮抗剂对海马神经元具有保护作用。Objective To observe the morphology of neuronal necrosis by kainic acid(KA) induced status epilepsy (SE) in rats, and to study the brain protective effect of Mg 2+.Methods 75 adult male Wistar rats were randomly divided into KA group, Mg 2+ group and normal saline group. SE was induced with KA for 3 hours, and the rats in Mg 2+ group were intraperitoneal injected magnesium sulfate before being injected KA. 72 hours later the rats were killed. We had all rat brain sections and observe the morphology of neuronal necrosis with microscope and electron microscope.Results In KA group, seizure was induced 16.1±4.7 min after injection of KA, but seizure delayed to 25.4±6.2 min in Mg 2+ group. There was a significant difference between two groups (P<0.05). Neuronal necrosis in hippocampus was found 72 hours later both in KA and Mg 2+ group. But neuronal damage was more slight in Mg 2+ group than in KA group.Conclusion Our results suggest that SE induced by KA results in neuronal necrosis in hippocampus, and Mg 2+ has protecting effects to the neurons of hippocampus as an exciting amino acid antagonism.

关 键 词：癫痫持续状态 海马 镁离子 坏死 

分 类 号：R742.1[医药卫生—神经病学与精神病学]