地衣芽孢杆菌GXN151的纤维素酶基因cel9A的序列分析及cel48A的定位  

Sequencing Analysis of Cellulase Gene cel9A and Localization of Gene cel48A from Bacillus Licheniformis GXN151

作　　者：张杰[1] 刘永生[1] 马庆生[2] 

机构地区：[1]中国农业科学院兰州兽医研究所,甘肃兰州730046 [2]中国科学院沈阳应用生态研究所,辽宁沈阳110015

出　　处：《沈阳农业大学学报》2004年第3期235-239,共5页Journal of Shenyang Agricultural University

基　　金：国家863计划2001AA214151;国家国际合作重点项目计划2002AA217121

摘　　要：运用底物筛选法从地衣芽孢杆菌GXN151的基因文库中筛选到3类表达羧甲基纤维素酶活性的克隆.对克隆pGXNP11的测序表明其含有纤维素酶基因cel9A和cel48A的部分序列,cel9A基因为1899bp,可编码含633个氨基酸的内切葡聚糖酶cel9A,cel9A的N-末端第21～456氨基酸形成家族9糖基水解酶催化功能域,第480～564氨基酸为家族3碳水化合物结合组件功能域,cel48A基因位于ceI9A基因的下游,两者可能共用一个操纵子.cel48A基因编码一个外切纤维素酶,通过染色体步移法将cel48A定位于4kb EcoRI片段上或10kb SalI片段上.Three types of clones expressing the carboxymethylcellulase activities were isolated from the genomic library of Bacillus licheniformis GXN151 by the substrate-based screening method. The DNA sequencing indicated the clone pGXNP11 harbors the cellu-lase gene ce19A and partial sequence of cel48A. The cel9A gene was 1899 bp, encoding family 9 endoglycohydrolase with 633 amino acids. The N-terminal amino acid 21～456 of Cel9A formed family 9 glycosyl hydrolase catalytic domain. Amino acid 480～565 was family 3 carbohydrate binding modules. The cel9A gene was found in an operon together with a partial cistron designated cel48A which encodes a product highly similar to bacterial exocellobiohydrolases belonging to family 48 of the glycosyl hydrolases. The cel48A gene was located on the 4 kb EcoRI fragment or 10 kb SalI fragment by the method of chromosome walking.

关 键 词：地衣芽孢杆菌 GXN151 纤维素酶 cel9A基因 序列分析 cel48A基因 基因定位 纤维素 

分 类 号：Q939.124[生物学—微生物学]