远交群体动态性状基因定位的似然分析 Ⅱ.模拟研究  

Maximum Likelihood Analysis for Mapping Dynamic Trait QTL in Outbred Population Ⅱ.Simulation

作　　者：杨润清 [1] 高会江 [2] 孙华 [2] Shizhong Xu  

机构地区：[1]上海交通大学农业与生物学院,上海,201101 [2]东北农业大学动物科学技术学院,哈尔滨,150030 [3]Department of Botany & Plant Science,University of California,Riverside CA 92521,USA

出　　处：《Acta Genetica Sinica》2004年第11期1254-1261,共8页

摘　　要：以远交设计群体为例 ,在推导出动态性状基因定位的似然法分析过程的基础上 ,选择 3阶Legendre多项式为子模型 ,采用Monte Carlo方法模拟研究了不同个体数、测定日频数、标记密度和QTL遗传贡献率对两种分析方法检测QTL效率的影响。每个因素都取高、中和低 3个水平 ,利用正交设计安排模拟因素试验组合。模拟试验结果表明 :高QTL遗传贡献率要比低QTL遗传贡献率的QTL在检测时需要较少个体数和测定日抽样 ;但不论QTL遗传贡献率多大 ,30 0以上的群体大小和 5 %以上的测定日频数都可以保证足够高的检测效率。个体数和测定日频数对动态性状QTL的分析和检测具有几乎相同的作用 ,而且相同样本含量条件下两者呈现互补的关系。就某个动态点的QTL检测而言 ,模拟试验也同时证明Based on the maximum likelihood analysis principle for dynamic trait QTL mapping,selecting three orders Legendre polynomial as sub-model,the effects of the individual number,the frequency of test day,the marker density and heritability on detecting power are investigated by Monte-Carlo simulation.Each factor is divided into three levels such as the high,middle and low,the combinations of which are arranged by using orthologal design.The simulating result shows that the high heritability needs less number of individuals and frequency of test day than the low one,but the number of individuals of more than 300 and the frequency of test day of 5% above can guarantee sufficient detecting power no matter what the heritability is like.The number of individuals has almost the same effect as the frequency of test day in dynamic traits QTL mapping and the number of individuals and the frequency of test day compensate each other under the same size of samples.As to the QTL detection for a dynamic point,simulation indicates that the presented dynamic trait QTL mapping method that focuses on the whole dynamic process is significantly superior to the classical mapping methods on each individual dynamic point.

关 键 词：动态性状 基因定位 LEGENDRE多项式 极大似然法 模拟 

分 类 号：Q348[生物学—遗传学]