骨肉瘤中p53、p21^(WAF1)、cyclinA蛋白的表达及其意义  

Significance of p53, p21^(WAF1) and cyclinA protein expression in osteosarcomas

作　　者：李扬[1] 梁惠珍[1] 罗灿峤[1] 邓家德[2] 朱全胜[1] 丘钜世[1] 

机构地区：[1]中山大学中山医学院病理学教研室,广州510089 [2]广州市第一人民医院检验科,广州510180

出　　处：《临床与实验病理学杂志》2004年第5期604-606,610,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金 (No 3 9970 2 89);中山医科大学"2 11工程"重点学科建设基金 (No 980 2 8)资助

摘　　要：目的　探讨骨肉瘤中 p5 3、p2 1WAF1、cyclinA蛋白的表达及相互关系。 方法　应用免疫组化方法对骨肉瘤组织及正常软组织中p5 3、p2 1WAF1、cyclinA的蛋白表达进行检测。结果　正常软组织中p5 3表达均阴性 ,2 8% (14 / 5 0 )骨肉瘤中可检测到p5 3蛋白的异常积累 ;正常软组织中均有不同程度的 p2 1WAF1蛋白的阳性表达 ,5 2 % (2 6 / 5 0 )骨肉瘤 p2 1WAF1阴性 ,骨肉瘤中p2 1WAF1的蛋白表达表现为p5 3蛋白依赖性的方式 ;正常组织中cyclinA为阴性 ,75 6 % (2 8/ 37)骨肉瘤中存在cyclinA蛋白过表达 ,cyclinA与p2 1WAF1的表达呈负相关 (r=- 0 874 ,P <0 0 1) ;p2 1WAF1蛋白的表达与骨肉瘤的分化呈正相关 (r =0 6 87,P<0 0 1)。结论　p5 3蛋白的异常积聚、p2 1WAF1的失表达及cyclinA的过表达参与了骨肉瘤失控的增生及肿瘤的形成。Purpose To investigate the significance of p53, p21 WAF1 and cyclinA protein expression in osteosarcomas and the relationship among these proteins. Methods Immunohistochemistry was performed to detect the expression of p53, p21 WAF1 and cyclinA in osteosarcomas and normal soft tissues. Results Normal soft tissues were negative for p53 protein, 28%(14/50) osteosarcomas contained abnormal p53 protein accumulation. All normal soft tissues expressed p21 WAF1 protein, 52% (26/50) osteosarcomas lack of p21 WAF1 protein expression. p21 WAF1 expressed in a p53-dependant way. Normal soft tissues expressed a low level of cyclinA, 75.6%(28/37) osteosarcomas over-expressed cyclinA protein. There was a negative correlation between cyclinA and p21 WAF1 protein expression(r= -0.874,P<0.01). p21 WAF1 expression was positively correlated with the differentiation of osteosarcoma(r=0.687,P<0.01). Conclusions Abnormal accumulation of p53, lack of p21 WAF1 and overexpression of cyclinA protein may contribute to the uncontrolled proliferation and oncogenesis of osteosarcoma.

关 键 词：骨肉瘤 P53 p21^WAH CYCLINA 基因表达 肿瘤形成 

分 类 号：R738.1[医药卫生—肿瘤]