TGF-β1在下颌骨牵张成骨中的局部表达及作用的实验研究  

Expreesion of TGF-β1 in mandibular distraction osteogenesis

作　　者：周诺[1] 梁飞新[1] 韦山良[1] 蒙宁[1] 覃迪生[1] 

机构地区：[1]广西医科大学口腔医学院,530021

出　　处：《实用口腔医学杂志》2004年第6期670-673,共4页Journal of Practical Stomatology

基　　金：国家自然科学基金 (30 1 60 0 88);广西科技厅自然科学基金 (990 53)资助项目

摘　　要：目的 :探讨下颌骨牵张成骨过程中的TGF β1在局部表达及其作用。 方法 :采用免疫组化定性及ELISA定量方法检测狗下颌骨牵张成骨中不同时间点TGF β1变化水平 ,并对照观察狗下颌骨骨不连中TGF β1的变化。结果 :ELISA定量检测中可见牵张组中骨痂组织TGF β1的表达在牵张中 6d ,牵张固定 2周 ,牵张固定 8周 ,均比骨不连组及正常组高 ;免疫组化染色可见阳性着色主要定位于牵张中 6d牵张区边缘活跃的成骨细胞及新生的血管壁周围 ,牵张固定 2周牵张区中间新形成的类骨质周围的活跃成骨细胞及基质 ,牵张固定 8周牵张区中间新形成与牵张力平行的骨小梁边缘的成骨细胞及基质。结论 :对狗下颌骨进行牵张成骨的过程中 ,机械的牵张力刺激 ,可使局部牵张区TGF β1持续较高表达 ;持续较高表达的TGFObjective:To investigate the expression of transforming growth factor β1(TGF-β1) in dog mandibular distraction osteogenensis (DO). Methods: Mandibular DO model was established in 12 dogs and nonunion model in another 12 dogs, 4 dogs were used as the no-treatment control. Tissue samples were obtained 6 d,2 and 8 weeks after operation respectively.Immunohistochemistry and enzyme-linked immunosorbent assay (ELISA) were used to determine the expressions of TGF-β1 in the samples.Results: TGF-β1 (ng/g) in control group was 1394.3±20.1,6 d after operation that in DO and nonunion groups was 1928.5±33.5(vs control,P<0.001) and 1831.5±23.9(vs control,P<0.001), 2 weeks after operation 1770.0±31(vs control,P<0.001) and 1407.6±21.8, 8 weeks after operation 1624.5±26.1(vs control,P<0.001) and 1102.3±29.4(vs control,P<0.001),respectively.Conclusion: The mechanical strain stimulates a high level expression of TGF-β1 in the bone tissue of distraction zone during DO.

关 键 词：牵张成骨 TGF-Β1 免疫组化 ELISA 

分 类 号：R782.23[医药卫生—口腔医学]