碱性成纤维细胞生长因子在人牙胚生长发育中的作用  

Expression of basic fibroblast growth factor in human tooth development

作　　者：谢家敏[1] 田卫东[1] 刘磊[1] 李罡[1] 

机构地区：[1]四川大学华西口腔医学院,610041

出　　处：《实用口腔医学杂志》2004年第6期724-726,共3页Journal of Practical Stomatology

基　　金：国家科技部重大基础研究前期专项资助课题 (编号 2 0 0 2CCC0 0 70 0 )

摘　　要：目的 :研究碱性成纤维细胞生长因子在人牙胚生长发育中的作用。方法 :选择胎龄分别为 7、8、10、14周自愿终止妊娠的新鲜人胚胎各 4例 ,制作牙胚切片 ,将蕾状期、帽状期和钟状期牙胚切片 ,做免疫组化检测。结果 :bFGF在蕾状期上皮细胞强染 ,外胚间充质细胞少量弱染色 ;帽状期 ,bFGF在上皮细胞呈整体弱染色 ,牙乳头细胞染色更弱 ;钟状期 ,bFGF在内釉细胞呈强染色 ,牙乳头近切缘的外层细胞亦呈阳性染色 ,星网状层少量细胞弱染色。结论 :bFGF对人牙胚上皮细胞的增殖和分化起着促进作用 ;在人牙胚的钟状期 ,bFGF在促进内釉上皮细胞向成釉细胞转化以及牙乳头细胞向成牙本质细胞转化中起重要作用 。Objective: To study the role of basic fibroblast growth factor (bFGF) during human tooth germ development.Method: bFGF expression was exmined with immmunohistochemical technique in bud stage,cap stage and bell stage of human tooth germ from aborted embryo of 7,8,10 and 14 weeks.Results: At the bud stage of human tooth development, bFGF was expressed in most dental epithelium cells,and few condensed mesenchymal cells.At the cap stage ,bFGF was weakly expressed.At the bell stage,bFGF was strongly expressed in the inner enamel epithelium layer and dental papillary cells near inner enamel epithelia,weakly in the stellate reticulum.Conclusion:During human tooth development,bFGF plays an important role in the proliferation and differentiation of dental epithelium.

关 键 词：碱性成纤维细胞生长因子 人牙胚 免疫组织化学 

分 类 号：R780.2[医药卫生—口腔医学]