日本血吸虫童虫冷冻保藏复苏后生活史循环的研究  

THE LIFE CYCLE STUDY OF SCHISTOSOMA JAPONICUM SCHISTOSOMULES AFTER CRYOPRESERVATING IN LIQUID NITROGEN AND ANABIOSIS

作　　者：林邦发[1] 汪英华[1] 田锷[1] 朱顺海[1] 石耀军[1] 张正达[1] 施福恢[1] 林矫矫[1] 

机构地区：[1]中国农业科学院上海家畜寄生虫病研究所

出　　处：《中国兽医寄生虫病》1994年第1期1-5,共5页Chinese Journal of Veterinary Parasitology

摘　　要：本文报告了日本血吸虫童虫经液氮冻藏复苏后的生活史循环的情况。童虫冻藏３６天和５０天后腹腔注射感染小鼠，亲代成虫回收率为９．０和８．９％。感染鼠肝内的子代虫卵孵出的子１代毛蚴感染钉螺，感染率为５９和６７％；平均每个钉螺第１次逸出的子１代尾蚴数为８００～９００条。子１代尾蚴感染小鼠后，其子１代成虫回收率为５５和５６％。合抱雌、雄成虫的体长、性腺的发育和虫体对宿主的致病力与正常途径感染者无明显差异。Some observation of the life cycle of S. japonicum schistosomules after cryopreservating in and recovering from liquid nitrogen was reported. The Schistosomules which were cryopreservated in liquid nitrogell for 36 and 50 days were used to inoculate Kunndng mice by i. p., the adult worm recovery rate were 9.0% and 8.9% respectively. The infecting rate of F1 miracidium, which were hatched from F1 eggs of cryopreservatad schistosomules infected mice liver, to intermediate host snail were 59% and 67%. The mean F1 cercaria escaped from each infected snail were 800 to 900 at the first exposure time. The F1 cercaria were used to infect mice, and the F1, adult worm recovery rate were 55% and 56%. NO difference were observed in the body length of paired-worms, the development of sexual glands and the pathogenicity of the parasite when using cryopreservated schistosomules to infect mice compared with those seen in the normal infecting route.

关 键 词：日本血吸虫 童虫 液氮 冻藏 复苏 生活史循环 

分 类 号：S852.735[农业科学—基础兽医学]