HSPC011基因在毛乳头细胞的表达及其cDNA克隆  

The Expression of Gene HSPC011in Dermal Papilla Cells of Hair Follicle and Its cDNA Cloning

作　　者：杨卫兵[1] 郝飞[1] 宋志强[1] 杨希川[1] 

机构地区：[1]第三军医大学西南医院皮肤科,重庆400038

出　　处：《中华皮肤科杂志》2003年第12期699-701,共3页Chinese Journal of Dermatology

基　　金：国家自然科学基金(30200249)

摘　　要：目的确定HSPC011基因在毛乳头细胞的表达,并克隆其cDNA。方法原位杂交技术证实HSPC011基因在毛乳头细胞表达;RT-PCR扩增目的基因并构建重组真核表达质粒pCI-neo/HSPCO11。结果细胞内mRNA原位杂交证实HSPC011基因在凝集性生长的毛乳头细胞表达,而在非凝集性生长的毛乳头细胞和人真皮成纤维细胞不表达;RT-PCR从毛乳头细胞RNA中扩增到约430bp目的基因片段;构建了重组质粒pCI-neo/HSPCO11,酶切分析和测序结果均表明目的片段与HSPC011基因相应序列完全一致。结论HSPC011基因在毛乳头细胞表达,其表达可能与毛乳头细胞的分化和功能状态有关。Objective To identify the expression of gene HSPC011(HSPC,hematopoietic stem/pro-genitor cell)in the dermal papilla cells of hair follicle and clone its cDNA.Methods The expression of gene HSPC011was confirmed by intracellular mRNA hybridization in situ;the objective cDNA was amplified by RT-PCR(reverse transcription PCR).Results By in situ hybridization,it was shown that gene HSPC011expressed in the coagulated dermal papilla cells,but not in the non-coagulated dermal papilla cells and the dermal fibroblast;The objective cDNA with a fragment of430bp was amplified by RT-PCR,and a recombi-nant eukaryotic expressing plasmid pCI-neo/HSPC011was constructed.By enzyme cutting and sequencing analysis,the objective cDNA was completely identical with gene HSPC011.Conclusion Gene HSPC011was clearly shown to express in the dermal papilla cells,and the expression of HSPC011was possibly related with the differentiation and functional status of the dermal papilla cells.

关 键 词：毛乳头细胞 表达 HSPC 目的基因 RT—PCR 扩增 真皮成纤维细胞 凝集性生长 CDNA克隆 RNA原位杂交 

分 类 号：R346[医药卫生—基础医学]