斑点分子杂交检测HPV感染与宫颈癌的关系  

Detection of Different HPV DNA(HPV6,11,16,18 type)in Cervical Lesions by DNA-DNA Dot Blot Hybridization

作　　者：孙汭[1] 孟祥金[1] 崔正言[1] 崔树龄[1] 李文[1] 

机构地区：[1]山东省医学科学院基础医学研究所,济南250001

出　　处：《临床与实验病理学杂志》1993年第4期244-246,共3页Chinese Journal of Clinical and Experimental Pathology

摘　　要：采用DNA-DNA分子杂交技术,对经病理组织学确诊的慢性宫颈炎、宫颈癌和正常宫颈的宫颈活检组织中HPV6、HPV11,HPV16、HPV18型DNA进行同源序列检测。结果表明HPV6、HPV11、HPV16、HPV18型DNA的检出率在正常宫颈均为0;在慢性宫颈炎分别为16.09%、12.64%、11.49%、3.45%;在宫颈癌组分别为3.96%、1.98%、46.53%、7.92%。在宫颈癌组HPV16、HPV18型均高于慢性宫颈炎组,HPV16型差异达到显著性(P<0.01)。经线性趋势分析,HPV16型与宫颈疾患存在显著的线性趋势关系(P<0.001),即HPV16型感染率升高,宫颈癌的发病亦升高。在慢性宫颈炎组HPV6、HPV11高于宫颈癌组。本结果进一步提示HPV16、HPV18型主要存在于癌组织,HPV6、HPV11型主要存在于宫颈良性疾病。To study the relationship betweendifferent types of HPV and cervical cancer,HPVDNA sequences(6,11,16,18)were detected byDNA-DNA dot blot hybridization in cervicalbiopsies which were pathologically diagnosed aschronic cervicitis,cervical cancer and normal ??cervical respectively.The results showed that thepositive rates of HPV6,11,16,18 type in nor-mal cervices,cervicitis and cervical cancer were0,0,0,0;16.09%,12.64%,11.49%,3.45%and 3.96%,1.98%,46.53%,7.92% respec-tively.HPV16,18 type in cervical cancer werehigher than that in cervicitis(x^2=27.16,1.70).There was significant difference betweencervical cancer and cervicitis for HPV 16. Lineartrend analysis showed that there was a lineartrend between HPV16 and cervical cancer.Thehigher the positive rates of HPV16,the higherincidence rate of cervical cancer(x^2=37.12,P<0.001).HPV 11,6 incervicitis were higherthan that in cervical cancer.It was further sug-gested that HPV16 played an important role incervical cancer and that HPV11,6 mainly exist-ed cervical benign lesions.

关 键 词：宫颈癌 乳头状瘤病毒 分子杂交 

分 类 号：R737.330.3[医药卫生—肿瘤]