DNA依赖蛋白激酶在人支气管上皮细胞癌变株和肺癌组织中的异常表达  

THE ABNORMAL EXPRESSION OF DNA-PKCS IN MALIGNANT TRANSFORMED CELL LINE OF HUMAN BRONCHIAL EPITHELIAL CELLS AND LUNG CANCERS

作　　者：隋建丽[1] 孙敬芬[1] 曹珍山[1] 周平坤 吴德昌[1] 

机构地区：[1]军事医学科学院放射医学研究所放射毒理研究室,北京100850

出　　处：《中国体视学与图像分析》2003年第3期141-145,共5页Chinese Journal of Stereology and Image Analysis

基　　金：国家"863"资助项目(2001AA221271);国家自然科学基金(30270423);军队杰出人才基金(01J006)

摘　　要：目的:探讨辐射诱发人支气管上皮细胞癌变株和肺癌组织中DNA依赖蛋白激酶(DNA dependent protein kinase, DNA-PK)的激酶活性或表达变化。方法:用Western blot和p53蛋白为特异性底物的磷酸化反应分别检测癌变细胞中DNA-PKcs表达和激酶活性,用免疫组化结合病理图像定量分析技术检测肺癌组织和癌旁组织中DNA-PKcs蛋白的表达情况。结果:a粒子辐射诱发的人支气管上皮细胞癌变株BERP35T-1的DNA-PKcs表达水平比亲本细胞BEP2D提高30%,激酶活性显著提高。所检测的14例非小细胞肺癌组织中DNA-PKcs蛋白表达水平普遍高于相对应的癌旁组织,两组之间有显著性差异(P < 0.05)。结论:非小细胞肺癌组织中DNA-PKcs表达增加,可能成为肺癌的一个生物标记物。OBJECTIVE: To investigate the changes of kinase activity or expression of the catalytic subunit of DNA-dependent protein kinase (DNA-PK)in a particle exposure-induced malignant transformed cell line of human bronchial epithelial cells as well as human lung cancer tissues. METHODS: Western blot was used to detect the expression of DNA-PKcs of culture cells. A peptide of P53 molecule was used as the specific substrate for the kinase activity assay of DNA-PKcs. The expression of DNA-PKcs in lung cancer tissues was examined by immunohistochemistry. RESULTS: The expression of DNA-PKcs in the a particle-induced malignant transformed cell line BERP35T-1 was increased by 30%, compared with the parental human bronchial epithelial cells BEP2D. The DNA-PKcs kinase activity of BERP35T-1 was significantly higher than that of BEP2D cells. The protein level of DNA-PKcs in all the 14 cases of detected non small cell lung cancer (NSCLC) tissues was significantly higher than that of the corresponding marginal normal tissues (P < 0.05). CONCLUSION: The expression of DNA-PKcs was increased in NSCLC tissues, and it could be recognized as a potential biomarker for lung cancer.

关 键 词：肺癌 a粒子 DNA—PKcs 基因表达:生物标记物 

分 类 号：R734[医药卫生—肿瘤]