LMP1促鼻咽癌细胞生长与端粒酶活性  

The potential relationship between growth promotion of nasopharyngeal carcinoma cells by latent membrane 1 and the activation of telomerase

作　　者：张素珍[1] 徐永[2] 黄培春[1] 陈锦[1] 蔡康荣[3] 黄河[1] 

机构地区：[1]广东医学院病理生理学教研室,湛江524023 [2]广东医学院生理学教研室,湛江524023 [3]广东医学院分析中心,湛江524023

出　　处：《基础医学与临床》2004年第4期440-444,共5页Basic and Clinical Medicine

摘　　要：为探讨EB病毒潜伏膜蛋白 1(LMP1)促鼻咽癌细胞生长作用与端粒酶活性的关系 ,用LMP1基因真核表达质粒转染鼻咽癌CNE1细胞 ;脂质体介导端粒酶反义核酸处理转染细胞 ;MTT法检测细胞增殖能力 ;原位杂交法检测端粒酶逆转录酶 (hTERT)mRNA表达 ;免疫组化法检测LMP1蛋白表达。结果显示 :转染LMP1基因的细胞增殖能力和hTERTmRNA表达水平均显著高于未转染和转染空载质粒的细胞 (均P <0 0 1)。经端粒酶反义核酸作用 4 8h ,LMP1基因转染细胞的细胞增殖能力、hTERTmRNA和LMP1蛋白表达水平均显著低于空白对照组 (均P <0 0 1) ,反义核酸和脂质体处理组上述各指标无显著变化 ;反义核酸组LMP1基因转染细胞的增殖能力和hTERTmRNA表达水平仍均显著高于未转染细胞和转染空载质粒的细胞 (均P <0 0 1)。以上结果提示 :EB病毒LMP1的促鼻咽癌细胞生长作用与端粒酶活性密切相关。To explore the relationship between the growth promotion of nasopharyngeal carcinoma(NPC) cells by Epstein-Barr Virus(EBV) latent membrane 1(LMP1) and the activation of telomerase. Eukaryotic expression plasmid with EBV-LMP1 gene was transfected into NPC CNE1 cells.After being treated with telomerase antisense oligodeoxynucleotide(ASODN),the proliferation of transfected cells was determined by MTT,and the expression of human telomerase reverse transcriptase(hTERT) mRNA and LMP1 protein were detected by in situ hybridization and immunohistochemistry respectively.The results showed that the proliferating ability and the expression of hTERT mRNA of LMP1-transfected cells were significantly higher than those of untransfected cells and mock vector-transfected cells (P<0.01). After treatment ASODN for 48h, the proliferation and the expression of hTERT mRNA and LMP1 protein in LMP1-transfected cells were significantly lower than those in the control groups(P<0.01). There was no significant changes in nonsense oligodeoxynucleotide groups and lipofectin groups. In ASODN groups, the proliferating ability and the expression of hTERT mRNA of LMP1-transfected cells were significently higher than those of untransfected cells and mock vector-transfected cells(P<0.01). It was proved that EBV LMP1 promoting NPC cells growth was corelated with telomerase activity.

关 键 词：LMP1基因 鼻咽癌细胞 反义核酸 端粒酶活性 细胞增殖能力 mRNA表达 转染细胞 真核表达质粒 生长 水平 

分 类 号：R739.6[医药卫生—肿瘤]