淀粉样β蛋白对神经组织一氧化氮水平的影响及褪黑素的拮抗作用  

Beta-amyloid for the levels of nitric oxide in nervous tissue and antagonistic role of melatonon

作　　者：叶静[1] 刘协和[2] 邓红[2] 

机构地区：[1]首都医科大学宣武医院神经内科,北京市100053 [2]四川大学华西医院精神科,四川省成都市610041

出　　处：《中国临床康复》2004年第34期7712-7713,共2页Chinese Journal of Clinical Rehabilitation

基　　金：国家"973"计划项目(G2000057005);中国博士后基金资助(99-10)~~

摘　　要：目的:观察淀粉样β蛋白(β-amyloid,Aβ)对体内神经组织和培养神经元一氧化氮水平的影响以及褪黑素的拮抗作用。方法:实验于2000-06/09在四川大学华西医院精神科实验室进行。①原代大鼠神经元培养,暴露不同剂量Aβ和褪黑素。②Aβ25～35海马内定向注射,褪黑素腹腔内注射。③比色法测定脑组织匀浆和神经细胞培养液一氧化氮水平和一氧化氮合酶(nitricoxidesynthase,NOS)活力。结果:Aβ可使脑组织和培养神经元一氧化氮水平增高,暴露Aβ浓度为0.5,1.0,10.0,20.0μmol/L组一氧化氮水平分别为(7.9±1.3),(9.2±1.5),(12.6±2.4),(14.1±1.4)μmol/L,与对照组(5.9±1.4)μmol/L比差异有显著性意义(P<0.01),同时Aβ使NOS活力升高(P<0.01);细胞培养液中一氧化氮水平和NOS活力与暴露Aβ剂量呈显著正相关(r=0.786,P<0.01);褪黑素可降低Aβ诱导的一氧化氮水平增加和NOS活力升高。结论:Aβ的神经毒性作用有一氧化氮途径参与,褪黑素可通过降低一氧化氮产生和NOS活力来部分对抗Aβ的毒性作用。AIM:To observe the impact of beta amyloid(Aβ) on nitric oxide(NO) levels of nervous tissue and cultured neuronal cells from rats and the antagonistic role of melatonin. METHODS:The experiment was carried out in the Laboratory of Psychiatric Department,West China Hospital of Sichuan University from June to November 2000.①Aβand melatonin were exposed to cultured neuronal cells from rats.②Aβ25-35 was microinjected into hippocampus and melatonin intraperitoneally injected into rats.③NO level and nitric oxide synthase(NOS) activity from brain tissue and cultured neuronal cell were measured by chromatometry. RESULTS:The Aβincreased the level of NO and the activity of NOS of brain tissue and cultured neuronal cell, showing a positive correlation with the concentration of Aβ(r=0.786,P< 0.01).The levels of NO were(7.9±1.3),(9.2±1.5),(12.6±2.4),(14.1±1.4)μmol/L respectively exposed to 0.5,1.0,10.0,20.0 μmol/L Aβ, which was significantly different from that of the control group[(5.9±1.4)μmol/L]. Melatonin decreased the level of NO and the activity of NOS induced by Aβ. CONCLUSION:NO is involved in the neurotoxic effects induced by Aβ; Melatonin is in part against the neurotoxicity of Aβby reducing the level of NO and the activity of NOS.

关 键 词：淀粉样Β蛋白 神经组织 一氧化氮 褪黑素 拮抗作用 神经毒性 

分 类 号：R741[医药卫生—神经病学与精神病学]