对马铃薯4x-2x,2x-4x及2x-2x杂种四倍体进行花药培养获得转育2n配子基因的双单倍体新个体  

DIHAPLOIDS WITH 2N GAMETES WERE OBTAINED BY ANTHER CULTURE OF TETRAPLOIDS FROM 4X-2X,2X-4X AND 2X-2X HYBRIDS IN POTATOES

作　　者：冉毅东[1] 

机构地区：[1]甘肃农业大学农业生物工程研究所,730070

出　　处：《马铃薯杂志》1993年第2期90-95,共6页Chinese Potato Journal

基　　金：农业部"八五"生物技术计划;甘肃省青年基金

摘　　要：利用3个2n配子材料(2x)在马铃薯(S.tuberosum L.)中进行4x—2x,2x—4x和2x—2x的杂交,获得了4个四倍体杂种材料;然后对它们进行花药培养,共得到32个双单倍体植株。检查其中23个植株,有2株是具5％以上2n花粉粒的双单倍体,1株是重组了2n卵基因的双单倍体。由此证明花药培养的倍性操作技术是转育马铃薯2n配子性状给双单倍体的有效方法之一。Four hybrids were obtained from 4x×2x, 2x×4x, and 2x×2x crosses by using two tetraploids (Solanum tuberosum L.) and three diploid materials which can produce 2n gamates. Thirty-two dihaploid plantlets were regenerated from embryoids of anthers in the four tetraploid hybrids by anther culture in vitro. Two of them could produce more than 5% of 2n pollen and one of them could produce 2n eggs. The results suggest that the ploid manipulation through sexual polyploidization and haploid induction is an effect method to get dihaploids with 2n gamates in potatoes.

关 键 词：马铃薯 杂种 四倍体 花药培养 

分 类 号：S532.035.3[农业科学—作物学]