端粒酶转录酶及其调节相关蛋白与增殖细胞核抗原在卵巢上皮性肿瘤中的表达及临床意义  

Expression of telomerase and telomerase associated-regulation protein and proliferating cell nuclear antigen in ovarian epithelial tumors

作　　者：高冬霞[1] 吕彧[2] 吕英志[3] 马彩红[4] 王玉萍[1] 张波[1] 廖松林[1] 

机构地区：[1]北京大学医学部病理学系,100083 [2]北京市朝阳医院病理科 [3]北京市第六医院病理科 [4]北京大学第三医院妇产科

出　　处：《中华病理学杂志》2003年第4期319-322,共4页Chinese Journal of Pathology

基　　金：国家自然科学基金资助项目 (3 0 170 3 64 );北京市科委基金资助项目(H0 10 2 10 47112 );北京大学人类疾病基因中心项目 (ZB0 7)

摘　　要：目的　探讨端粒酶转录酶 (hTERT)及端粒酶调节相关蛋白 (TRAP)与增殖细胞核抗原(PCNA)在卵巢上皮性肿瘤中的表达和临床意义。方法　收集 10 6例卵巢上皮性肿瘤 (恶性 5 4例 ,交界性 33例 ,良性 19例 )的临床资料 ,行hTERT、TRAP和PCNA 3种抗体的免疫组织化学标记链霉素卵白素生物素 (LSAB)法染色。对 87例恶性和交界性患者进行了随访 ,4 5例获结果 ,随访时间为 2～6 0个月。结果　hTERT蛋白的表达在良性 (4/ 19)和交界性 (90 9% ,30 / 33)以及良性和恶性(94 4 % ,5 1/ 5 4 )间的差异均有显著性 (P均 <0 0 0 1) ,TRAP蛋白的表达在良性 (4/ 15 )和恶性(77 8% ,2 8/ 36 )间的差异有显著性 (P <0 0 0 1) ;hTERT和TRAP蛋白的表达在卵巢癌Ⅰ、Ⅱ期和Ⅲ、Ⅳ期两组病例中的差异均无显著性 (P >0 0 5 ,P >0 3)。PCNA的表达在良性 (6 9± 5 9) %和交界性 (2 6 4± 17 8) %、良性和恶性 (5 1 8± 2 2 1) %以及交界性和恶性间的差异均有显著性 (P <0 0 1,P <0 0 0 1,P <0 0 5 )。 33例交界性患者全部存活 ,5 4例恶性患者中 35例 (6 4 8% )有转移 (包括 5例淋巴结转移 ) ,4例 (7 4 % )死亡。结论　hTERT和TRAP蛋白的表达与卵巢上皮性肿瘤的良恶性有关 ,但与其临床分期无关 ;Objective To investigate expression and significance of hTERT?telomerase associated-regulation protein (TRAP) and proliferating cell nuclear antigen (PCNA) in ovarian epithelial tumors. Methods 106 specimens of ovarian epithelial tumors and their clinical history were collected, including 54 cases of malignancy, 33 borderline cases and 19 benign tumor cases. Immunohistochemical staining for hTERT, TRAP and PCNA were performed. Follow-up information was obtained for 45 of 87 cases (malignancy in 54 and borderline malignancy in 33). Results The expression of hTERT was significantly different between benign (4/19) and borderline (90.9%, 30/33) cases, benign and malignant (94.4%, 51/54) cases (P<0.001), as was the expression of TRAP between benign (4/15) and malignant (77.8%, 28/36) cases (P<0.001). The expression of hTERT and TRAP was not higher in stage Ⅲ, Ⅳ ovarian cancer patients than in stageⅠ and Ⅱ(P>0.05, P>0.3). The expression of PCNA between benign (6.9±5.9)% and borderline (26.4±17.8)% cases, benign and malignant (51.8±22.1)% cases, and borderline and malignant cases were different, and were statistically significant (P<0.01,P<0.001,P<0.05). 33 cases of borderline malignance are all survive. In 54 cases of malignance, 35 of them have metastasis (64.8%), including 5 cases of lymph nodes metastasis. 4 of them died (7.4%). Conclusions The expression of hTERT and TRAP is associated with the malignant degree of ovarian cancer, but does not correlate with stage. The expression of TRAP resembles hTERT, which may be a new tumor-associated gene. Telomerase activity is positively associated with PCNA.

关 键 词：端粒酶转录酶 增殖细胞核抗原 卵巢上皮性肿瘤 基因表达 端粒酶调节相关蛋白 

分 类 号：R737.31[医药卫生—肿瘤]