单纯疱疹病毒Ⅰ型在兔角膜内潜伏和活化的实验研究  

A study of the latency and reactivation of HSV-1 in the rabbit cornea

作　　者：谢立信[1] 董晓光[1] 史伟云[1] 张德如[1] 李绍伟[1] 孟莉[2] 

机构地区：[1]山东省医学科学院眼科研究所,青岛市266033 [2]青岛市第二人民医院眼科,266033

出　　处：《眼科》1994年第2期107-111,共5页Ophthalmology in China

摘　　要：在健康新西兰兔角膜上建立HSK模型,待其病变稳定期,一组行自体穿透性角膜移植,另一组与未感染HSV-1的兔角膜行交叉穿透移植,拆线后行肾上腺素离子透入拟使潜伏病毒活化。离子透入后的植片抗原检测均为阳性,透射电镜检查显示病毒感染征象,植片角膜基质细胞培养发生HSV-1自发感染。实验结果显示角膜组织是HSV-1的另一个潜伏感染处和复发源地。Herpes simplex keratitis models were created with healthy New Zealand rabbits. During the quiescent period, one group of the rabbits were performed autograft penetrating keratoplasty and another group cross penetrating keratoplasty with noninfeeted rabbits. After removal of the sutures, epinephrine iontophoresis was done for reactivation of the virus, and all the corneal buttons were stained positive of HSV-1 antigens. Transmission electron microscopy revealed signs of viral infection and culture of corneal graft keratoeytes also demonstrated HSV-1 infection, suggesting that the cornea was a site of HSV-1 latency and source of recurrence.

关 键 词：角膜 单纯疱疹病毒 潜伏感染 病毒激活 角膜移植 

分 类 号：R772.21[医药卫生—眼科]