脑缺血再灌注后脑组织c－fos基因表达与丹参的影响  

Effect of Radix Salviae Miltiorrhizae(RSM)on c-fos Gene Expression in Rat Brains after Cerebral Ischemia and Reperfusion

作　　者：吴卫平[1] 李振洲[1] 匡培根[1] 

机构地区：[1]北京解放军总医院神经内科神经介质实验室

出　　处：《中国神经免疫学和神经病学杂志》1995年第1期1-3,T001,共4页Chinese Journal of Neuroimmunology and Neurology

摘　　要：采用线栓法制成大鼠大脑中动脉缺血再灌注模型，用地高辛精标记ｃ－ｆｏｓ探针进行原位杂交。结果示缺血再灌注鼠栓塞侧皮层及海马ｃ－ｆｏｓ基因表达显著增多，图像分析灰阶值为１１８．６±５．１，对侧为１５９．６±３．１（Ｐ＜０．００１）。丹参组栓塞侧皮层及海马ｃ－ｆｏｓ基因表达亦增多，灰阶为１３５．００±２．０５，对侧为１６７．００±２．００（Ｐ＜０．００１）。丹参组与缺血再灌注组比较，栓塞侧丹参组ｃ－ｆｏｓ基因表达显著低于缺血再灌组（Ｐ＜０．０５），而两组栓塞对侧比较无显著差异。本实验表明，脑缺血再灌注后脑组织ｃ－ｆｏｓ基因表达显著增多，丹参能部分抑制缺血后ｃ－ｆｏｓ基因的表达，这可能是其治疗缺血性脑血管病的机理之一。The local cerebral ischemia and reperfusion model of rats was made with thread embolism of middle cerebral artery.After 90 min ischemia following 30 min reperfusion,c-fos mRNA expression in rat brain was investigatied with in situ hybridization in ischemia following reperfusion and RSM-treated group.The results showed that c-fos gene expression in cerebral cortex and hippocampus of ischemic sides was significantly increased as compared to nonischemic sides (P<0. 001). Although c-fos gene expression in RSM treated-rat was also increased,it was significantly lower than that of ischemia following reperfusion group(P<0.05).The results indicated that c-fos gene expression in rat brain can be induced by cerebral ischemia following reperfusion and RSM may partly play a role in inhibition of c-fos gene expression during ischemia and reperfusion.

关 键 词：C-FOS基因 表达 丹参 栓塞 后脑组织 脑缺血再灌注 皮层 探针 

分 类 号：R743[医药卫生—神经病学与精神病学] R285.5[医药卫生—临床医学]