钙/钙调素依赖的蛋白激酶Ⅱ-α在Alzheimer病老年斑的表达  

The expression of calcium/calmodulin-dependent protein kinase Ⅱ in the senile plaques in Alzheimer disease

作　　者：陈贵海[1] 王月菊 周江宁[3] 

机构地区：[1]安徽医科大学第一附属医院神经内科,安徽合肥230022 [2]安徽省老年病研究所 [3]中国科技大学生命科学院

出　　处：《中国神经科学杂志》2004年第6期441-445,共5页

基　　金：科技部 973项目 (G19990 5 40 0 7);科技部国际合作项目 (2 0 0 1CB71110 1)

摘　　要：目的　 探讨钙 /钙调素依赖的蛋白激酶Ⅱ (CaMKⅡ ) α在Alzheimer病 (AD)老年斑形成中的作用。方法　 选择 10例女性AD患者及 10例年龄、性别等匹配的正常老年人的海马切片 ,进行CaMKⅡ α免疫组织化学和CaMKⅡ α与β 淀粉样蛋白 (Aβ)或胶质纤维酸性蛋白 (GFAP)双标免疫荧光组织化学研究。 结果　CaMKⅡ α正常分布于神经元胞质、轴突 ,但在AD患者的海马 ,CaMKⅡ α沉积于神经元外并形成大小不等的斑块样结构。其中 ,大部分CaMKⅡ α斑块样沉积存在于主要由Aβ组成的老年斑中 ,只有那些很小的CaMKⅡ α沉积斑不含Aβ。在弥散型斑块中 ,CaMKⅡ α沉积撒落在未融合的Aβ沉积中。在经典型斑块中 ,交互出现的CaMKⅡ α和Aβ沉积围绕着均质融合的Aβ核心。老年斑周围反应增生性星形胶质细胞不过度表达CaMKⅡ α ,且与CaMKⅡ α沉积间无明确关联。结论　CaMKⅡ α沉积可能参与了AD患者老年斑形成 ,星形胶质细胞可能不参与CaMKⅡ α沉积。Objective To understand the role of calcium/calmodulin-dependent protein kinase II (CaMKII-α) in the formation of senile plaques in patients suffering from Alzheimer disease (AD). Methods The paraffin sections of the hippocampuses of 10 female AD patients were stained with immunohistochemistry and double-labeling immunofluresence methods. Results The CaMKII-α deposited in the extraneurons and formed the plaques with different sizes. Most of the CaMKII-α plaques located in the senile plaques principally containing β-amyloid, only just those very small CaMKII-α plaques did not contain β-amyloid. In the classic plaques, the β-amyloid core were surrounded by the CaMKII-α deposits, whereas the CaMKII-α deposits were fully overlapped or mixed with β-amyloid in the diffuse plaques. There was no over-expression of CaMKII-α in the reactive astrocytes surrounded the senile plaques. Conclusion CaMKII-α may be close associated with the formation of the senile plaques in AD, and the astrocytes may be not attributable to the deposition of CaMKII-α.

关 键 词：ALZHEIMER病 老年斑 钙-钙调素依赖的蛋白激酶Ⅱ-α Β-淀粉样蛋白 星形胶质细胞 

分 类 号：R749.16[医药卫生—神经病学与精神病学]