硝酸羟胺急性毒性和诱导染色体损伤的初步研究  

Study on the Acute Toxicity and the Genetic Damage of Chromosome in Mice After Exposure to Hydroxylammonium Nitrate

作　　者：贾庆军[1] 杨录军[1] 刘晋祎[1] 周燕虹[1] 刘胜学[1] 曹佳[1] 

机构地区：[1]第三军医大学军事预防医学院卫生毒理教研室,重庆400038

出　　处：《癌变．畸变．突变》2004年第6期359-361,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：军队指令性课题(No.180.3160-O1L015);国家杰出青年基金资助项目(No.30125037);军队杰出青年基金资助项目(No.01J014)

摘　　要：背景与目的:探讨硝酸羟胺的急性毒性和半数致死剂量(LD50)以及对染色体的影响,为其安全使用提供依据。材料与方法:小鼠腹腔注射不同浓度的硝酸羟胺溶液,观察小鼠死亡率和脏器的病理改变;计数小鼠骨髓细胞微核率和染色体畸变率,评价硝酸羟胺对小鼠的毒性作用程度。结果:小鼠的LD50 24 h为186 mg/KG(177-197 mg/kg);14 d为183 mg/kg(174-194mg/kg);肉眼未见脏器有病理改变。小鼠腹腔注射硝酸羟胺实验组微核发生率、染色体畸变率与对照组相比差异有显著性。结论:按化学物质的急性分级标准,硝酸羟胺属中等毒级别,腹腔注射硝酸羟胺可诱导小鼠染色体损伤。BACKGROUND & AIM: To explore the acute toxicity and LD50, and study the genetic damage of bone marrow cells induced by Hydroxylammonium Nitrate. MATERIAL AND METHODS: After celiac administration of HAN in mice, the mortality and the physiological changes were observed; Chromosome aberration test, micronucleus test in mouse were used. RESULTS: The LD50 of HAN in male on 24 hour and 14 day was 186 mg/kg(185-187 mg/kg) and 183 mg/kg (182- 184 mg/kg), respectively. No pathological change of the organs was found. There were significant findings in chromosome aberration and micronucleis rates in experimental groups compared with those of the negative control group(P < 0.05). CONCLUSION: According to the standard of grading of acute toxicity,HAN belongs to the moderate class. The genetic damage of bone marrow cells can be induced by Hydroxylammonium Nitrate.

关 键 词：硝酸羟胺 急性毒性 LD50 骨髓细胞微核 染色体畸变 

分 类 号：R994.6[医药卫生—毒理学]