胰腺癌组织中神经激肽3受体的表达及组织学定位  

The expression and localization of neurokinin-3 receptor in pancreatic carcinoma

作　　者：石欣[1] 高乃荣[1] 郭庆明[2] 霍明东[1] 汤文浩[1] 

机构地区：[1]东南大学附属中大医院普外科,南京210009 [2]东南大学附属中大医院病理科,南京210009

出　　处：《中华普通外科杂志》2004年第11期678-680,共3页Chinese Journal of General Surgery

基　　金：国家人事部留学回国人员科研启动基金(编号:7690004027)

摘　　要：目的研究神经激肽3受体(NK3R)在胰腺癌组织中mRNA和蛋白水平的表达,以及该受体在胰腺癌中的组织学定位。方法应用逆转录聚合酶链反应技术检测正常胰腺和胰腺癌组织中NK3R的mRNA水平,应用Westernblot检测其蛋白的表达水平,应用免疫组织化学技术进行组织学定位。结果与正常胰腺相比,胰腺癌组织中NK3RmRNA过度表达,胰腺癌组织中NK3R蛋白也过度表达,正常胰腺组织NK3R蛋白表达为11±05,胰腺癌组织为146±36,P<001。免疫组织化学检查结果显示正常胰腺中有较弱的NK3R表达,胰腺癌的导管细胞、神经纤维、炎性细胞中均有较强的NK3R表达。结论胰腺癌组织中NK3R的表达水平明显上升。Objective To investigate the expression of neurokinin 3 receptor (NK 3R) in normal pancreas and pancreatic cancer, and to study the localization of this receptor in pancreatic cancer Methods The expression of NK 3R mRNA was investigated using reverse transcription polymerase chain reaction (RT PCR) in normal pancreas and pancreatic cancer tissues The protein level of NK 3R was investigated by Western blot Immunohistochemistry was used to localize expression site of NK 3R Results NK 3R mRNA was overexpressed in pancreatic cancer tissue when compared with normal pancreas NK 3R protein was 1 1±0 5 in normal pancreas and 14 6±3 6 in pancreatic cancer, P <0 01 Immunohistochemistry showed that weak NK 3R immunoreactivity was observed in acinar and ductal cells in normal pancreas, while in pancreatic cancer samples, strong NK 3R immunoreactivity was present in most cancer cells, in intrapancreatic nerves and ganglia, and inflammatory cells Conclusion NK 3R was overexpressed in pancreatic carcinoma, its clinical implication in terms of carcinogenosis needs to be investigated

关 键 词：胰腺癌组织 NK 正常 过度表达 受体 神经激肽 表达水平 组织学定位 蛋白 RNA 

分 类 号：R735.9[医药卫生—肿瘤]