食管鳞癌中鸟氨酸脱羧酶mRNA、内皮抑素mRNA表达和血管生成相关性研究  

Investigation on the mRNA expressions of ornithine decarboxylase and endostatin in esophageal squamos cell carcinoma and their correlation with angiogenesis

作　　者：苗新普[1] 李建生[1] 师晓天[2] 

机构地区：[1]郑州大学第一附属医院消化科,450052 [2]安阳市肿瘤医院胸外科

出　　处：《中华消化杂志》2004年第11期668-671,共4页Chinese Journal of Digestion

基　　金：河南省教育厅自然科学基础研究计划项目(19993 2 0 0 0 3 ;2 0 0 13 2 0 0 0 3 2 )

摘　　要：目的　研究鸟氨酸脱羧酶(ODC)mRNA、内皮抑素mRNA和微血管密度(MVD)在食管鳞癌中的表达及相关关系,探讨ODC在食管鳞癌血管生成中的可能作用,及其与肿瘤浸润和转移的关系。方法　用半定量RT PCR法测定4 1例食管鳞癌患者癌组织(T)和相应癌旁组织(N)中的ODCmRNA和内皮抑素mRNA表达,求得各自T/N值;同时用免疫组化方法测定癌组织和癌旁正常组织MVD的T/N值。结果　在4 1例患者中,39例ODCmRNA的T/N值>1.0 ,占95 .1% :4 0例MVD的T/N值>1.0 ,占97.6 %。36例内皮抑素mRNA的T/N值>1.0 ,占87.8%。ODCmRNA、内皮抑素mRNA和MVD与食管鳞癌的肿瘤大小、浸润深度和淋巴结转移有关,ODCmRNA、内皮抑素mRNA表达与肿瘤分化程度有关。ODCmRNA的T/N值与MVD的T/N值呈正相关,与内皮抑素mRNA的T/N值呈负相关,内皮抑素mRNA的T/N值与MVD的T/N值呈负相关。结论　ODC与食管鳞癌血管生成和肿瘤浸润转移密切相关。ODC过表达可能是通过抑制内皮抑素而促进血管生成,从而促进食管鳞癌的浸润和转移。Objective To observe the mRNA expressions of ornithine decarboxylase(ODC), endostatin and microvessel density (MVD) in esophageal squamous cell carcinoma (ESCC) and their correlations, and to explore the mechanisms of ODC in facilitating angiogenesis, its correlation with invasion a nd metastasis of the carcinoma. Methods Semi-quantitative RT-PCR method was used to detect the mRNA expressions of ODC and endostatin in 41 paired surgically resected specimens of ESCC (T)and their adjacent tissue(N), a nd the T/N ratio was calculated.Immunohistochemistry was used to detect the MVD by anti-CD 34 monoclonal antibody in ESCC tiss ue and corresponding adjacent tissue. Results Of 41 cases, the T/N ratio of ODC mRNA was greater than 1.0 in 39 cases (95.1%) and the T/N ratio of MVD was greater than 1.0 in 4 0 cases ( 97.6%). The mRNA expressions of ODC and endostatin, and MVD in esophageal specimens we re significantly correlated with tumor sizes, lymph node metastasis and adventit ia invasion. The mRNA expressions of ODC and endostatin were correlated with differentiation status of tumor. T/N value of ODC mRNA was positively correlated with T/N value of MVD, and nega tively correlated with the T/N value of endostatin mRNA. While the T/N value of endos tatin mRNA was negatively correlated with the T/N value of MVD. Conclusion ODC is closely related to tumor angiogenesis, invasion and metastasis of ESCC. It is possible that ODC overexpr ession may promote tumor angiogenesis by suppressing endostatin expression, which may be o ne of the mechanisms that ODC facilitates invasion and metastasis of ESCC.

关 键 词：内皮抑素 食管鳞癌 MRNA表达 MVD 血管生成 ODC 鸟氨酸脱羧酶 负相关 进食 

分 类 号：R735.1[医药卫生—肿瘤]