去磷酸化RB蛋白在乳腺癌细胞凋亡中的作用  

The role of dephosphorylated RB protein in human breast cancer cell apoptosis

作　　者：王文武[1] 欧阳学农[1] 姜浩[2] 曹建国[2] 

机构地区：[1]南京军区福州总医院肿瘤科,福建福州350025 [2]南华大学附属第一医院肿瘤科,湖南衡阳421001

出　　处：《医学研究生学报》2004年第12期1059-1061,F031,共4页Journal of Medical Postgraduates

基　　金：湖南省卫生厅基金课题资助项目 (批准号 :2 0 0 10 Y5 8)

摘　　要：目的 :研究去磷酸化RB蛋白表达在人乳腺癌细胞凋亡中的作用。　方法 :以乳腺癌细胞株MCF 7/S(化疗敏感细胞株 )和MCF 7/Adr(耐多柔比星细胞株 )为研究对象 ,采用免疫细胞化学法检测多柔比星 (ADR)作用前、后RB蛋白的表达状态 ;应用流式细胞术检测ADR诱导乳腺癌细胞凋亡程度。　结果 :加ADR前两种细胞胞核中的磷酸化RB蛋白均为阳性 ,去磷酸化RB蛋白均为阴性。经不同浓度ADR处理后 ,随着其浓度的增加 ,MCF 7/S细胞去磷酸化RB蛋白表达量逐渐增高 ,而MCF 7/Adr细胞去磷酸化RB蛋白表达量无明显增高。流式细胞术检测细胞凋亡率和细胞周期 ,结果表明ADR诱导MCF 7/S细胞凋亡作用比MCF 7/Adr强 (P <0 .0 1)。　结论 :去磷酸化RB蛋白表达与MCF 7/S细胞凋亡呈正相关 ,而与MCFObjective: To investigate the role of the expression of dephosphorylated RB protein for cell apoptosis in human breast cancer. Methods: In our works, human breast cell lines (MCF-7/S,the chemosensitive cell line and MCF-7/Adr, the chemoresistent cell line)were evaluated. The expressive levels of dephosphorylated RB protein were detected with immunocytochemistry. Apoptosis rates were determined by flow cytometry(FCM). Results: Before treatment of ADR, phosphorylated RB protein was positive in two cell lines, but dephosphorylated RB protein was negative; After treatment of different concentration ADR, when the concentration of ADR was increased, expression of dephosphorylated RB protein elevated accordingly in MCF-7/S, but no significant change in MCF-7/Adr. Apoptosis and cycle was detected by FCM assays shows ADR induced apoptosis of MCF-7/S more than MCF-7/Adr(P<0.01). Conclusion: There were positive correlation the expression levels of dephosphorylated RB protein and the apoptosis of MCF-7/S cells for Chemosensitivity, but no significant correlation in MCF-7/Adr.

关 键 词：去磷酸化RB蛋白 乳腺癌 细胞凋亡 多柔比星 

分 类 号：R737.9[医药卫生—肿瘤]