前列腺癌相关基因表达谱分析  

Analysis of related gene expression in prostate carcinoma by cDNA microarray

作　　者：习小庆[1] 胡映波[1] 潘正跃[1] 吴勇[1] 黄红卫[1] 刘芳明[1] 葛根[1] 彭瑞元[1] 应康[2] 谢毅[2] 

机构地区：[1]江西医学院第二附属医院泌尿外科,330006 [2]上海复旦大学遗传学研究所

出　　处：《中华泌尿外科杂志》2004年第12期800-803,共4页Chinese Journal of Urology

基　　金：国家自然科学基金项目 (3 9760 0 72 )

摘　　要：目的　探讨前列腺癌发生发展中相关基因群的表达意义。　方法　应用含有 4 36 6条人类全长基因cDNA基因芯片研究一组前列腺癌及正常前列腺组织基因表达谱的差异性。同时进行生物信息学分析。　结果　在前列腺癌与正常前列腺组织间存在差异表达的基因 2 87条 ,未知基因16 5条 ,已知基因 12 2条。已知基因中有显著差异表达基因 5 6条 ,其中上调基因 2 0条 ,下调基因 36条。　结论　生物信息分析显示 :显著差异表达基因与肿瘤的发病机理可能存在相关性。Objective To evaluate the role of differentially expressed genes in human prostate carcinoma initiation and progression. Methods cDNA microarray chips which consist of a set of 4366 human genes were used to investigate the gene expression pattern of samples from human prostate cancer and normal prostate tissues. Results Of 4366 genes,287 genes differentially expressed in prostate carcinoma and normal prostate tissues were screened out,including 165 unknown genes and 122 known genes. Among the 122 known genes we further identified 20 up-regulated and 36 down-regulated genes. Conclusions Our results suggest that significantly differential expression of genes may be associated with the pathogenesis and progression of the prostate cancer.

关 键 词：前列腺癌 基因表达谱 生物信息学 发病机理 

分 类 号：R737.25[医药卫生—肿瘤]