应用G-CSF对不同人群外周血CD_(34)^+细胞和T细胞亚群影响的动态变化  

Effects of G-CSF on peripheral blood CD_( 34)_^+cells and T ly mphocyte subsets in different populatins

作　　者：王存邦[1] 欧英贤[1] 白海[1] 潘耀柱[1] 魏亚明[1] 欧建锋[1] 慕海霞[1] 王晓静[1] 

机构地区：[1]兰州军区兰州总医院血液科,甘肃兰州730050

出　　处：《医师进修杂志》2004年第12期16-17,20,共3页Journal of Postgraduates of Medicine

摘　　要：目的　探讨重组人粒细胞集落刺激因子 (G -CSF)对健康人及处于完全缓解的恶性血液病患者的外周血CD+34细胞和T细胞亚群的影响。方法　对 12例健康人和 16例自体外周血干细胞移植患者 ,以G -CSF 15 0 μg 12h ,皮下注射 ,连用 6d。应用流式细胞仪测定外周血CD+34 和T细胞亚群。结果　 (1)应用G -CSF前 ,两者的外周血CD+34细胞分别为 (0 .39± 0 .2 7) %和 (0 .4 7± 0 .2 5 ) % ,两者之间无明显差异 (P >0 .0 5 )。应用 72h后 ,CD+34 细胞分别达(1.2 9± 0 .6 4 ) %和 (1.4 7± 0 .95 ) % ,较前均有明显增加 (P <0 .0 1) ,两者之间无明显差异 (P >0 .0 5 )。应用 96h后 ,CD+ 34 细胞达高峰 ,分别为 (1.4 1± 0 .73) %和 (1.5 8± 0 .83) % ,两者之间无明显差异 (P >0 .0 5 ) ,但较应用G -CSF前差异显著 (P <0 .0 1)。 (2 )无论是否应用G -CSF ,恶性血液病患者的T细胞亚群比例均处于倒置状态 ,而健康人T细胞亚群则比例正常。随两者应用G -CSF后CD+34 细胞逐渐增加 ,T细胞亚群变化不明显。结论　健康人及处于完全缓解的恶性血液病患者 ,在应用G -CSF后 ,外周血CD+ 34 细胞比例逐渐增加并于 96h后达到高峰 ,T细胞亚群无明显变化。Objective To observe effects of G-CSF on peripheral blood CD+ 34 cells and T lymphocyte subsets in different populations.Methods CD+ 34 cells and T lymphocyte subsets were measured by flow cy tometry in 12 cases of donors and 16 cases of patients of APBSCT who were injected G-CSF 150 μg/12 h for 6 days.Results (1)Before using G-CSF,the CD + 34 cells of patients and donors were (0.39±0.27)% and (0.47±0.25)%.Th ere wer e no obvious difference between them(P>0.05).After dosing G-CSF fo r 72 h,they were (1.29±0.64)% and (1.47±0.95)% respectively.There were a s ignificant increase (P<0.01),but no obvious difference between two g roups(P>05). After 96 h, the peak percentages were reached to (1.4 1± 0.73)% and (1.58±0.83)% respectively. There were significant difference ( P<0.01),but no significant difference between the two group(P >05).(2)CD 4/CD 8 in the donors was normal but was abnormal in the patien ts . There were no obvious changes in T lymphocyte subsets after dosing G-CSF. Conclusion Peripheral blood CD+ 34 cells of donors and patient s reached peak levels after using G-CSF for 96 h and no obvious changes in T lymphocyte subsets.

关 键 词：重组人粒细胞集落刺激因子 外周血 CD^+34细胞 T淋巴细胞亚群 

分 类 号：R733.7[医药卫生—肿瘤]