大蒜素对结肠癌Lovo细胞端粒酶活性、环氧化酶-2及核因子-κB的影响  

Effect of Allicin on the Telomerase Activity,Cyclooxygenase-2 and Nuclear Factor-kappa B of Colon Cancer Lovo Cell Line

作　　者：关文明 范钰[2] 林庚金[2] 钱立平[2] 许祖德[3] 黄富春 

机构地区：[1]河南省漯河市第二人民医院内科,漯河462000 [2]复旦大学附属华山医院消化科,上海200040 [3]上海医学院病理学系,上海200032

出　　处：《复旦学报（医学版）》2004年第6期597-599,共3页Fudan University Journal of Medical Sciences

摘　　要：目的　探讨大蒜素对结肠癌细胞的作用机制。方法　采用不同浓度的大蒜素处理结肠癌Lovo细胞后 ,分别应用四唑盐比色试验、端粒重复扩增微孔板杂交法、RT PCR和Westernblot方法检测环氧化酶 2mRNA和蛋白水平的表达 ,采用凝胶迁移率法检测核因子 κB活性。结果　大蒜素对结肠癌Lovo细胞增殖具有较强的抑制作用。大蒜素能够抑制端粒酶活性 ,且这种作用呈浓度及时间依赖。同时发现 ,大蒜素能抑制环氧化酶 2mRNA和蛋白水平的表达及抑制核因子 κB活性。结论　大蒜素能够抑制结肠癌细胞的恶性增殖 ,其机制与抑制端粒酶活性、抑制环氧化酶 2基因的表达及抑制核因子Purpose To identify the action mechanism of Allicin on colon carcinoma Lovo cell line. Methods After colon cancer Lovo cell were treated with Allicin , MTT assay, TRAP-microtiter plate hybridization assay ,RT-PCR and Western blot, electrophoretic mobility shift assay were used to detect the suppression rate ,the mRNA and protein of cyclooxygenase-2 gene, nuclear factor -κB of colon cancer Lovo cell. Results Allicin had a remarkable inhibition on colon cancer Lovo cell line. It could suppress the telomerase activity and expression of mRNA and protein ,and nuclear factor-κB. The effects were concentration-and time -dependent. Conclusions Allicin can inhibit the growth of huamn colon cancer Lovo cell with inhibiting telomerase activity and expression of cyclooxygenase-2 ,and nuclear factor -kappa B.

关 键 词：核因子-KB LOVO细胞 环氧化酶-2 端粒酶活性 结肠癌 抑制 表达 RNA 基因 微孔板杂交 

分 类 号：R735[医药卫生—肿瘤] R737[医药卫生—临床医学]