EGFR mRNA和COX-2 mRNA在NSCLC组织中表达的临床意义  

Expression and Clinical Significance of Epidermal Growth Factor Receptor Gene and Cyclooxygenase-2 Gene in Non-small Cell Lung Cancer

作　　者：高志强[1] 周彩存[1] 

机构地区：[1]同济大学附属肺科医院肿瘤科,上海200433

出　　处：《同济大学学报（医学版）》2004年第6期484-487,共4页Journal of Tongji University(Medical Science)

摘　　要：目的　探讨表皮生长因子受体 (epidermalgrowthfactorreceptor,EGFR)mRNA及环氧合酶 2 (cyclooxygenase 2 ,COX 2 )mRNA在非小细胞肺癌 (non smallcelllungcancer,NSCLC)组织中表达的临床意义。 方法　抽取 4 8例NSCLC组织中的RNA ,应用逆转录聚合酶链反应 (RT PCR)技术检测其中EGFRmRNA及COX 2mRNA的表达。结果　在 2 8例肿瘤组织中存在EGFRmRNA的表达 ,T3 4期的患者的EGFRmRNA表达率显著高于T1 2期的患者 (P <0 .0 5 ) ;在 36例肿瘤组织中存在COX 2mRNA的表达 ,肺腺癌COX 2mRNA的表达率显著高于肺鳞癌(P <0 .0 5 ) ,COX 2mRNA的表达率与 pTNM分期关系密切 (P <0 .0 5 ) ,COX 2mRNA的表达强度与原发肿瘤、淋巴结转移及 pTNM分期的关系密切 (P <0 .0 5 )。COX 2mRNA阳性表达的患者其EGFRmRNA表达率显著高于COX 2mRNA阴性表达的患者 (P <0 .0 5 ) ;EGFRmRNA与COX 2mRNA两者有显著相关性 (相关系数rs=0 .380 7,P <0 .0 5 )。结论　NSCLC中EGFRmRNA和COX 2mRNA的表达在NSCLC的发生、发展和转移中具有重要意义 ,二者可能有协同作用 ,可作为评估NSCLC进展及判断预后的重要指标。Objective To investigate the clinical significance of expression of epidermal growth factor receptor(EGFR) and cyclooxygenase-2(COX-2) gene in non-small cell lung cancer(NSCLC).Methods After RNA was extracted from 48 cases of NSCLC tissue,expressions of EGFR mRNA and COX-2 mRNA were examined by reverse transcription-polymerse chain reaction(RT-PCR).Results Expression of EGFR mRNA was observed in 28 cases of NSCLC.Expression of EGFR mRNA in T3-4 stage patients was higher than that in T1-2 stage patients(P<0.05).Expression of COX-2 mRNA was observed in 36 cases of NSCLC.Expression of COX-2 mRNA was significantly higher in adenocarcinoma than in squamous carcinoma(P<0.05).Expression of COX-2 mRNA was associated with TNM stages.The expression intensity of COX-2 mRNA was associated with primary tumor,lymph nodes metastasis and TNM stages(P<0.05).Expression of EGFR mRNA in patients who was positively expressed COX-2 mRNA was higher than others.Expression of EGFR mRNA was tightly correlated with the expression of COX-2 mRNA(r s=0.3807,P<0.05).Conclusion The expressions of EGFR mRNA and COX-2 mRNA may play an important role in occurrence,development and metastasis of NSCLC.EGFR mRNA and COX-2 mRNA may be useful biomarkers to determine prognosis and clinical strategies.

关 键 词：肺肿瘤 表皮生长因子受体 环氧合酶-2 基因表达 逆转录聚合酶链反应 

分 类 号：R734.2[医药卫生—肿瘤]