槲皮素恢复柔红霉素在白血病耐药细胞K562/ADM和HL-60/ADM中的分布  

Restorative Effect of Quercetin on Subcellular Distribution of Daunorubicin in Multidrug Resistant Leukemia Cell Lines K562/ADM and HL-60/ADM

作　　者：蔡讯[1] 陈芳源[2] 韩洁英[2] 顾春红[2] 钟华[2] 欧阳仁荣[2] 

机构地区：[1]上海交通大学附属第六人民医院肿瘤内科,上海200233 [2]上海第二医科大学附属仁济医院血液科上海血液学研究所白血病研究室,上海200001

出　　处：《癌症》2004年第12期1611-1615,共5页Chinese Journal of Cancer

基　　金：上海市科委基金资助项目(No.994319228);上海市卫生局基金资助项目(No.98418)~~

摘　　要：背景与目的:槲皮素是一种天然黄酮类中药成分,具有多种生理活性,最近发现其有逆转白血病细胞耐药的作用,本研究旨在探讨槲皮素恢复柔红霉素在白血病耐药细胞的分布从而达到逆转耐药的机制。方法:通过MTT体外药敏法检测槲皮素对柔红霉素的增敏作用并确定逆转的浓度范围,作用于K562/ADM、HL-60/ADM细胞及相应敏感株K562和HL-60,借助激光共聚焦显微镜观察槲皮素作用前后柔红霉素在亚细胞水平的分布变化。结果:20～40μmol/L槲皮素在体外能明显提高柔红霉素对K562/ADM和HL-60/ADM的敏感性,恢复柔红霉素在亚细胞水平的分布,使其回归细胞核内,从而逆转多药耐药。结论:黄酮类中药槲皮素能够成为蒽环类药物治疗白血病中有效的化疗增敏剂。BACKGROUND & OBJECTIVE:Quercetin,a widely distributed natural flavonoid with a variety of biological functions,can reverse multidrug resistance (MDR) in leukemia according to recent researches. This study was to investigate the mechanism of quercetin restoring subcellular distribution of daunorubicin (DNR)in multidrug resistant leukemia cell lines,K562/ADM and HL 60/ADM,and reversing their MDR. METHODS: MTT cell viability assay was used to verify the sensitization of DNR by quercetin in K562/ADM and HL 60/ADM cells,and determine the reverse concentration extent,confocal laser scanning microscope was used to observe the subcellular distribution of DNR in K562/ADM and HL 60/ADM cells,and relevant sensitive cell lines, K562/S and HL 60/S,before and after quercetin exposion. RESULTS: Compared with K562/S and HL 60/S cells,20-40 μmol/L of quercetin in vitro remarkably enhanced the sensitivity of K562/ADM and HL 60/ADM cells to DNR,restore the subcellular distribution of DNR,so as to reverse MDR. CONCLUSION: quercetin could be a candidate of effective multidrug resistance reversing agent in leukemia chemotherapy.

关 键 词：槲皮素 白血病 多药耐药 逆转 

分 类 号：R733.7[医药卫生—肿瘤]