中药脊髓Ⅰ号对脊髓厚片Ca^(2+)荧光强度的影响  

Effects of Chinese herb on the fluorescent intensity of the Ca^(2+) in gray matter in spinal cord

作　　者：高秀来[1] 刘霞[1] 马育平[1] 陈亚亮[1] 宋一志[1] 景鹏[1] 韩凤岳[2] 苏静[2] 刘卫东[2] 张平[2] 常超英[2] 刘恩[2] 

机构地区：[1]首都医科大学解剖学教研室,北京110001 [2]中国中医研究院骨伤科研究所

出　　处：《解剖学研究》2004年第1期32-34,i002,共4页Anatomy Research

基　　金：北京市教委 1997年基金资助项目

摘　　要：目的　探讨中药脊髓Ⅰ号对脊髓厚片Ca2 + 荧光强度的影响。方法　将 33只Wistar大鼠随机分为 3组 :下胸段脊髓半横断组 (损伤组 )、对照组和中药脊髓Ⅰ号治疗组。快速处死大鼠 ,取下胸段脊髓 ,切脊髓厚片 ,用Fluo 3荧光染料孵育 ,激光共聚焦显微镜观察。结果　损伤组、对照组和中药治疗组左侧Ca2 + 荧光强度分别为 12 6 3± 3 2 7、13 34± 3 5 1和 12 89±3 31,右侧分别为 2 1 6 8± 3 6 9、14 72± 2 12和 12 97± 3 6 0 ,损伤组脊髓厚片损伤侧Ca2 + 荧光强度显著升高 ,治疗组两侧灰质Ca2 + 荧光强度无显著差异。结论　 (1)损伤侧Ca2 + 荧光强度显著升高 ;(2 )中药脊髓Ⅰ号对Ca2 + 荧光强度的升高有一定抑制作用。Objective To study the effect of Chinese herb on the fluorescent intensity of the Ca 2+ in spinal cord after spinal cord hemisection.Methods The experimented rats were divided into three groups:the control group,the injured group and the group administered Chinese herb,the later two groups were operated spinal cord right-hemisection.We mensurated fluorescence intensity of the Ca 2+ in the spinal cord slice by laser scanning microscopy.Results The up-regulation of the fluorescence intensity of the Ca 2+ in injured side,and the fluorescent intensity of the Ca 2+ in the group administered Chinese herb was heightened not as much as in the injured group. Conclusions (1)the fluorescence intensity of the Ca 2+ was heightened after injury;(2)Chinese herb can help gray matter cells escape from increasing of Ca 2+,which benefit to cells survival.

关 键 词：脊髓 CA^2+ 中药 升高 损伤 胸段 对照组 荧光强度 荧光染料 激光共聚焦显微镜 

分 类 号：R285.5[医药卫生—中药学]