CTGF对角膜成纤维细胞生物学功能的影响  

Effect of connective tissue growth factor on biological function of corneal fibroblasts

作　　者：黄琼[1] 胡燕华[1] 李琦涵[2] 王炯[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院眼科,武汉430022 [2]中国医学科学院医学生物研究所

出　　处：《眼科研究》2004年第6期569-572,共4页Chinese Ophthalmic Research

摘　　要：目的 观察结缔组织生长因子(CTGF)对体外培养的角膜成纤维细胞增殖、向肌成纤维细胞转化和合成细胞外基质的影响。 方法 体外培养兔角膜成纤维细胞,经0.5、5、50和500 ng／mL CTGF处理24 h后,分别进行增殖细胞核抗原(PCNA)免疫组化染色、银染核仁组成区染色(AgNOR)、平滑肌肌动蛋白(α-SMA)免疫组化染色以及Ⅲ型胶原(CA-Ⅲ)、纤维连接蛋白(FN)和层黏蛋白(LM)免疫组化染色,检测CTGF对角膜成纤维细胞增殖、向肌成纤维细胞转化和细胞外基质合成的影响。 结果 与对照组相比,0.5、5、50和500 ng／mL的CTGF能剂量依赖性地促进角膜成纤维细胞PCNA表达,增加核仁银染颗粒数,促进α-SMA的表达,同时能促进角膜成纤维细胞CA-Ⅲ、FN和LM的合成(P<0.01)。结论 一定质量浓度的CTGF可能在角膜损伤修复过程中发挥重要作用。Objective To study the effects of connective tissue growth factor (CTGF) on the proliferation, transdifferentiation into myofibroblasts and extracellular matrix synthesis of cultured corneal fibroblasts. Methods Cultured passage 2 rabbit corneal fibroblasts were treated with 0. 5 ,5 ,50 and 500 ng/mL CTGF for 24 hours. The proliferating cell nuclear antigen (PCNA) and silver staining of nucleolar organizer region (AgNOR) ,α-smooth muscle actin (α-SMA) and collagen-Ⅲ, fibronectin and laminin were evaluated using immunohistochemistry staining. Results Compared with control group,0. 5 ,5 ,50 and 500 ng/mL CTGF increased AgNOR and α-SMA expressions in corneal fibroblasts (P < 0. 01 ) and promoted PCNA, collagen-Ⅲ,fibronectin and laminin synthesis of corneal fibroblasts in a dose-dependent manner (P < 0. 01). Conclusion CTGF may play critical roles during corneal wound healing process.

关 键 词：CTGF 角膜成纤维细胞 免疫组化染色 Α-SMA 肌成纤维细胞 FN 细胞外基质 银染 核仁 生物学功能 

分 类 号：R770.3[医药卫生—眼科]