检测IgH和TCRγ基因重排对NHL分期参考价值的探讨  

Clinical value of detecting immunoglobulin heavy chain and T-cell receptor ? chain gene rearrangements in non-Hodgkin's lymphoma by polymerase chain reaction

作　　者：刘迎[1] 武淑兰[1] 薛海鹏[1] 朱平[1] 

机构地区：[1]北京医科大学第一医院血液研究室,北京100034

出　　处：《临床肿瘤学杂志》1998年第1期4-7,共4页Chinese Clinical Oncology

摘　　要：目的:探讨以IgH和TCRγ基因重排为标志对NHL患者临床分期的参考价值.方法:多聚酶链反应(PCR)技术结合限制性酶谱分析患者骨髓和淋巴结细胞DNA IgH和TCRγ基因重排的克隆性.结果:形态学无骨髓浸润的23例NHL患者发现6例(26.1%)具单克隆基因重排.结论:IgH和TCRγ基因重排作为分子标志对形态学检查不能确认骨髓浸润的NHL患者的分期诊断有一定参考价值.Purpose:To investigate the usefulness of the detection of IgH and TCR gene rearrangements in staging of NHL patients. Methods: DNA samples of NHL patients were analyzed by polymerase chain reaction and restriction enzyme analysis. Results:Monoclonal IgH and/or TCR r rearrangements were detected in 6 cases among 23 NHL bone marrow samples without morphologic involvement. Conclusion:The detection of monoclonal IgH and TCR Y gene rearrangements in bone marrow may be of value for the staging of NHL patients.

关 键 词：Γ基因重排 NHL TCR 患者 骨髓浸润 参考价值 分期诊断 分子标志 克隆性 形态学检查 

分 类 号：R733[医药卫生—肿瘤] R392[医药卫生—临床医学]