表皮生长因子对人视网膜色素上皮细胞增生及移行的影响  

Effects of epidermal growth factor on the proliferation and migration of culture d human retinal pigment epithelial cells

作　　者：闫峰[1] 惠延年[1] 王雨生[1] 郭长梅[1] 

机构地区：[1]第四军医大学西京医院眼科研究所,陕西西安710032

出　　处：《眼科新进展》2004年第6期417-421,共5页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助 (编号 :39970 780 )~~

摘　　要：目的　观察表皮生长因子 (epidermalgrowthfactor,EGF)及血清对体外培养的人视网膜色素上皮 (retinalpigmentepithelium ,RPE)细胞增生及移行的影响。 方法　对培养的人 3～ 6代RPE细胞采用MTT比色法观察不同浓度的EGF(0 .1、1、10、5 0、10 0 μg·L-1)及血清对人RPE细胞增生的影响 ;应用RPE细胞损伤模型 ,计数进入缺损区的细胞 ,定量观察EGF及血清对RPE细胞移行的影响。结果　在细胞增生的实验中 :无血清组 ,10～ 10 0 μg·L-1EGF达到最佳刺激浓度 ,其增生率为 81.8% ;5 0mL·L-1血清组 ,1～10 μg·L-1EGF的刺激作用最强达到 12 2 .7% ;无血清组与 5 0mL·L-1血清组间有显著性差异 (P <0 .0 0 1) ,且 5 0mL·L-1血清可明显促进人RPE细胞的增生 (P <0 .0 0 1)。在细胞的移行实验中 :无血清组 ,1～ 10 0 μg·L-1EGF可促进RPE细胞移行 ,但作用较弱 ;10 0mL·L-1血清组中 1～ 10 μg·L-1EGF促移行作用最强达 4 38.9% ;1～ 10 0 μg·L-1EGF的促进基础RPE细胞移行能力 (最强为 36 % )低于 10 0mL·L-1血清诱导的RPE细胞的移行能力 (强度为 14 7% ) ;无血清组与 10 0mL·L-1血清组间有显著性差异 (P <0 .0 0 1)。结论　EGF对体外培养的人RPE细胞具有浓度依赖性促增生和移行的作用 ;Objective To examine the effect of epidermal gr o wth factor (EGF) and fetal bull serum (FBS) on the proliferation and migration o f cultured human retinal pigment epithelial (RPE) cells. Methods Cultured human RPE cells of 3th～6th passages were stud ied by colorimetric assay for cellular growth and survival (MTT assay) to invest igate the effects of EGF (0.1、1、10、50、100μg·L -1 ) and FBS on proliferation of human RPE cells;We used an in vitro wound healing model and counted th e number of cells that had entered the denuded area to assess the effects of EGF and FBS on the migration of human RPE cells. Results EGF stimulated proliferation and migration of cultured human RPE cells in a concentration-dependent manner.The maximal proliferation rate of RPE cells was 81.8% at 10～100μg·L -1 EGF in DMEM and 122.7% at 1～10μg·L -1 EGF in 50mL·L -1 FBS DM EM;There was significantly difference in DMEM groups and 50mL·L -1 FBS DME M groups (P<0.001),moreover, 50mL·L -1 FBS notably induced human RPE c ells proliferation (P<0.001).And 1～100μg·L -1 EGF slight ly stimulated human RPE cells migration;The maximal migration rate of RPE cells was 438.9% at 10～100μg·L -1 EGF in 10% FBS DME M;Ten percent FBS induced human RPE cel ls migration (the migration rate,147%) stronger than 1～100μg·L -1 EGF did alone(the maximal migration rate,36%);The migration rate of 100mL ·L -1 F BS DMEM groups had significantly higher than that of DMEM groups(P<0.001) . Conclusion EGF can stimulate proliferation and migration of cultured human RPE cells in a concentration-dependent manner. FBS can also induce RPE cells migration and proliferation, as well as cooperate with EGF.

关 键 词：增生性玻璃体视网膜病变 视网膜色素上皮细胞 细胞培养 表皮生长因子 细胞增生 细胞移行 

分 类 号：R774.1[医药卫生—眼科]