层粘连蛋白促进兔角膜内皮细胞bcl2表达的最适浓度测定  

Optimum concentration of laminin on the bcl-2 expression of rabb it's corneal endothelial cells

作　　者：朱晓谦[1] 吴静[1] 王彦平[2] 

机构地区：[1]暨南大学医学院眼科研究室,广东广州510632 [2]暨南大学病理生理学教研室

出　　处：《眼科新进展》2004年第6期426-428,共3页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助(编号 :39870 855) ;广东省重点科研基金资助 (编号 :2KM0 52 0 3S)~~

摘　　要：目的　探索层粘连蛋白促进兔角膜内皮细胞bcl 2表达的最适浓度。方法　给于体外培养的兔角膜内皮细胞以不同浓度的层粘连蛋白 ,采用酶联免疫技术找到最适作用浓度。结果　在一定的浓度范围内 (2 0～ 6 0mg·L-1)层粘连蛋白能够促进家兔角膜内皮细胞bcl 2的表达 ,并且成量效依赖关系 ,在 6 0mg·L-1时有最佳的促进作用。结论　层粘连蛋白可以促进兔角膜内皮细胞bcl 2基因表达的作用 ,最适作用浓度为 6 0mg·L-1。Objective To investigate optimum concentration of laminin(LN)on the bcl-2 ex pression of rabbit's corneal endothelial cells. Methods Cultured rabbit's corneal endothelial cells were given LN of different concentrations and the ELISA was ch osen to measure OD.We compared the OD and got the optimum concentration of LN o f each group. Results Within certain concentration (20～60 mg·L -1 ), the LN could promote bcl-2 gene expression in rabbit's corneal endothelial cells in a dose- dependent manner with the optimal promote effect at 60 mg·L -1 . Conclusion LN could pr omote bcl-2 gene expression in rabbit's corneal endothelial cells,and its opti mal promote effect is at 60 mg·L -1 .

关 键 词：最适浓度 层粘连蛋白 BCL-2基因 兔角膜内皮细胞 酶联免疫技术 

分 类 号：R772[医药卫生—眼科]