葡萄糖作辅助碳源对Klebsiella pneumoniae发酵甘油生产1,3-丙二醇的影响  

Effect of Glucose as Cosubstrate on 1,3-Propanediol Fermentation by Klebsiella pneumoniae

作　　者：程可可[1] 凌宏志 张丽莉 孙燕[1] 刘德华[1] 

机构地区：[1]清华大学化学工程系,北京100084 [2]黑龙江辰能生物工程有限公司,黑龙江哈尔滨150001

出　　处：《过程工程学报》2004年第6期561-566,共6页The Chinese Journal of Process Engineering

基　　金：'十五'国家科技攻关计划资助项目(编号:2001 BA708B01-04)

摘　　要：在Klebsiella pneumoniae发酵甘油生产1,3-丙二醇的种子培养及发酵实验中,考察了葡萄糖作辅助碳源对菌体生长及1,3-丙二醇生成的影响. 结果表明,在种子培养期,以葡萄糖和甘油为混合碳源可缩短种子培养周期;在批次发酵和流加发酵中,葡萄糖作辅助碳源可使1,3-丙二醇产率及得率明显提高,但不同的葡萄糖加入方式对产率及得率促进的效果不同. 在初始发酵培养基中添加5 g/L葡萄糖、并在4～40 h进行葡萄糖与甘油混合液连续流加的条件下,1,3-丙二醇浓度、产率及得率较单一甘油为底物的流加发酵结果分别提高41.2%, 38.6%和8.3%.The effect of glucose as cosubstrate for the seed culture and fermentation of 1,3-propanediol by Klebsiella pneumoniae with glycerol as substrate was investigated. The results showed that the mixed substrate of glycerol and glucose for the seed culture could shorten the seed culture period. When adding glucose as cosubstrate, the productivity and yield of 1,3-propanediol were increased greatly in batch and fed-batch fermentation, but different glucose feed strategies could lead to different results. Compared with the 1,3-propanediol fed-batch fermentation production with glycerol as the only substrate, the 1,3-propanediol concentration, productivity and yield increased by 41.2%, 38.6% and 8.3% respectively when 5 g/L glucose was added to the initial fermentation media and a mixture of glycerol and glucose was fed during 4~40 h.

关 键 词：1 3-丙二醇 Kklebsiella PNEUMONIAE 葡萄糖 

分 类 号：TQ923[轻工技术与工程—发酵工程]