神经干细胞增殖分化相关蛋白在皮质酮大鼠中的变化  

Changes of related proteins of neural stem cell differentiation and proliferation in rats treated with corticosterone

作　　者：高唱[1] 王景周[1] 吴伟康[2] 

机构地区：[1]第三军医大学大坪医院野战外科研究所神经内科,重庆400042 [2]中山大学中山医学院中西医结合研究所,广东广州500089

出　　处：《中国病理生理杂志》2004年第12期2272-2275,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30271652)

摘　　要：目的观察神经干细胞(NSC)增殖分化相关蛋白在皮质酮(CORT)大鼠NSC增殖分化中的作用。方法利用Westernblot方法检测NSC增殖分化相关蛋白变化。结果造模后30d和60d、CORT大鼠NSC增殖分化相关蛋白Notch1、hes5、Mash1、NeuroD表达水平显著低于正常对照组,尤以Mash1、NeuroD更显著(P<001);右归饮可明显增加Notch1、hes5、Mash1、NeuroD表达水平(P<001)。结论CORT显著抑制NSC增殖分化相关蛋白表达水平,右归饮能部份拮抗CORT对NSC增殖分化相关蛋白表达的抑制。Notch和bHLH家族可能与右归饮促进NSC增殖分化作用有关。AIM: To explore the changes of related proteins of neural stem cell differentiation and proliferation in rats treated with corticosterone (CORT). METHODS: Western blot method was used to investigate the changes of related protein of neural stem cell differentiation and proliferation. RESULTS: Compared with the normal control group, related proteins (Notch1, hes5, Mash1 and NeuroD) of neural stem cell differentiation and proliferation were significantly decreased in the CORT rats on the day 30 and 60, especially for Mash1 and NeuroD (P<0.01). Compared with the model group, the levels of Notch1, hes5, Mash1 and NeuroD in CORT rats were significantly increased on the day 30 and 60 after treated with the You Gui-Yin, a Chinese medicine (P<0.01). CONCLUSIONS: CORT effectively decreases the levels of Notch1, hes5, Mash1 and NeuroD, which are related to the differentiation and proliferation of neural stem cells. You Gui-Yin partly reverses the inhibition of CORT. Notch family and bHLH transcription factor are probably involved in the neurogenesis of You Gui-Yin. [

关 键 词：皮质酮 右归饮 神经干细胞 NOTCH BHLH 

分 类 号：R363[医药卫生—病理学]