葡萄糖转运体3在脑缺血/再灌注后半影区表达的变化及意义  

Changes of glucose transporter-3 gene expression in penumbra following ischemia and reperfusion of brain in rats

作　　者：李方成[1] 陶宗玉[1] 刘安民[1] 李军亮[1] 张蕲[2] 吴中华[1] 林吉惠[1] 

机构地区：[1]中山大学附属第二医院神经外科,广东广州510120 [2]华中科技大学同济医学院病理生理系,湖北武汉430030

出　　处：《中国病理生理杂志》2004年第12期2276-2279,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.39900048);广东省自然科学基金资助项目(No.010721)

摘　　要：目的研究大鼠局灶性脑缺血不同缺血时间和不同再灌注时间的脑梗塞体积比、皮质半影区葡萄糖转运体3(GLUT3)转录水平和蛋白水平的表达。方法用线栓法复制大鼠局灶性脑缺血模型,用KontronIBAS25全自动图像分析系统检测脑梗塞体积比;剥取缺血半影区皮质组织,采用反转录-聚合酶链反应(RT-PCR)测定GLUT3mRNA水平的变化;用免疫组织化学方法半定量测定GLUT3蛋白水平的变化。结果脑缺血1h后再灌注组的脑梗塞体积明显小于缺血3h再灌注组梗塞体积。GLUT3自3h即开始升高,24h到达高峰,1周时仍高于假手术对照组;缺血3h再灌注组在3h有一下降点,然后升高,24h到高峰,1周时接近正常水平。GLUT3蛋白水平的表达与mRNA相符合。结论GLUT3在缺血半影区的表达上调,可能是机体对缺血/再灌注的保护性反应。AIM: To investigate the volume percentage of infarct and expression level of glucose transporter-3 (GLUT3) transcription and protein at different ischemic time points and different reperfusion time points in rat focal cerebral ischemic penumbra. METHODS: Focal ischemic models of middle cerebral artery occlusion (MCAO) in rats were made by inserting nylon thread. Brain samples were harvested from ischemic penumbra. Infarct volume was analyzed quantitatively by Kontron IBAS 2.5 image auto-analyses system. The change of GLUT3 mRNA was assessed by RT-PCR, and the expression of GLUT3 protein was assessed by immunohistochemistry. RESULTS: The infarction volume in MCAO 1 h/R group was obviously smaller than that in MCAO 3 h/R group. GLUT3 began to ascend at 3 h in MCAO 1 h/R group, reached to climax at 24 h and remained higher than normal at 1 week. In contrast, in the MCAO 3 h/R group, GLUT3 had a descent at 3 h. Later on, it ascended rapidly, and reached climax at 24 h. At 1 week, it approached to normal. The expression level of GLUT3 protein corresponds with that of mRNA. CONCLUSION: GLUT3 expression is up-regulated in the penumbra region after focal cerebral ischemia, it may be a protective reaction against ischemia/reperfusion injury. [

关 键 词：脑缺血 再灌注 半影区 单糖转运蛋白质类 免疫组织化学 

分 类 号：R743[医药卫生—神经病学与精神病学]