肺鳞状细胞癌中EGFR和ERK2共表达与ERK2激活的关系  

Relationship between coexpression of EGFR and ERK2 and activation of ERK2 in lung squamous cell carcinomas

作　　者：闫文生[1] 宋兰英[2] 梁启万[1] 方嬿[1] 

机构地区：[1]中山大学肿瘤防治中心实验研究部,广东广州510060 [2]南方医科大学病理学教研室,广东广州510515

出　　处：《中国病理生理杂志》2004年第12期2299-2302,共4页Chinese Journal of Pathophysiology

基　　金：广州市科技计划项目(No.2002J1-C0121)

摘　　要：目的从蛋白和mRNA水平研究EGFR和ERK2在肺鳞状细胞癌(SCC)中的共表达及其与ERK2激活之间的关系。方法分别采用Westernblotting和反转录PCR(RT-PCR)对20例SCC及其配对正常肺组织中的EGFR和ERK2蛋白和mRNA的表达进行检测,ERK2的活性采用免疫沉淀和激酶反应进行测定。结果在配对的正常肺组织中EGFR和ERK2的mRNA和蛋白的表达几乎检测不到或仅有少量表达,但在20例肺癌组织中表达显著上调,其中肺癌组织中ERK2蛋白的表达比正常肺组织上调3-5倍;肺癌组织中ERK2的活性也比正常肺组织显著增加。结论在SCC中,EGFR和ERK2的蛋白共表达发生了上调,主要归因于其基因转录水平的激活,并可能最终导致肺癌组织中ERK2活性的增加。AIM: To evaluate the relationship between coexpression of EGFR and ERK2 on the level of protein and mRNA and activation of ERK2 in lung squamous cell carcinomas (SCC). METHODS: Twenty cases of lung squamous cell carcinomas and their paired normal lung tissues were studied. The expression of mRNA and protein of EGFR and ERK2 was measured by reverse transcription PCR (RT-PCR) and Western blotting, respectively. The activity of ERK2 was detected by immunoprecipitation and kinase reaction. RESULTS: The results showed that the expression of EGFR mRNA and protein in paired normal lung tissues was hardly detected by RT-PCR and Western blotting, respectively, but in 20 cases of lung SCCs their expression was upregulated significantly. Compared with normal lung tissues, the expression of ERK2 mRNA and protein in lung cancer tissues was also upregulated significantly, the latter increased about 3-5-fold, and the activity of ERK2 in lung SCCs was also markedly activated. CONCLUSIONS: These data highlight that the overexpression of ERK2 and EGFR protein occurs simultaneously in lung SCCs, which mainly results from the increasing level of gene transcription, the increased activity of ERK2 in lung cancer tissues perhaps attributes to the upregulation of coexpression of EGFR and ERK2. [

关 键 词：肺 肿瘤 鳞状细胞 受体 表皮生长因子 有丝分裂索激活蛋白激酶类 信号传递 

分 类 号：R730[医药卫生—肿瘤]