Resolution of 2-Octanol via Lipase-catalyzed Enantioselective Acetylation in Organic Solvents  

作　　者：WANGZhi LIZheng-qiang YuDa-hai WENGLiang LIUMing ZHANGGui-rong CAOShu-gui 

机构地区：[1]KeyLaboratoryforMolecularEnzymologyandEngineeringofEducationalMinistry,JilinUniversity,Changchun130023,P.R.China [2]LifeScienceandTechnologyInstituteofJilinUniversity,Changchun130023,P.R.China

出　　处：《Chemical Research in Chinese Universities》2004年第5期575-578,共4页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.2 0 0 72 0 11and2 0 2 72 0 17)

摘　　要：The lipases from different sources were screened for their ability to catalyze the resolution of 2-octanol in organic solvents with vinyl acetate as the acylating reagent. The medium effect has been studied on the irreversible transesterification with varying water activity(a_w). The influence of vinyl acetate concentration on it has also been investigated. Under the optimal conditions, the enantiomeric ratio(E value) of pseudomonas fluorescence lipase(PFL) exceeded 200 with an enantiomeric excess(e.e.) of S-2-octanol above 99% at a 51% degree of conversion.The lipases from different sources were screened for their ability to catalyze the resolution of 2-octanol in organic solvents with vinyl acetate as the acylating reagent. The medium effect has been studied on the irreversible transesterification with varying water activity(a_w). The influence of vinyl acetate concentration on it has also been investigated. Under the optimal conditions, the enantiomeric ratio(E value) of pseudomonas fluorescence lipase(PFL) exceeded 200 with an enantiomeric excess(e.e.) of S-2-octanol above 99% at a 51% degree of conversion.

关 键 词：Lipase RESOLUTION Activity ENANTIOSELECTIVITY 2-OCTANOL 

分 类 号：O623.411[理学—有机化学]