动态浊度法定量检测葛根素注射液中细菌内毒素  

Application of the Turbidimetric-kinetic Method for the Measurement of Endotoxin in Puerarin Injection

作　　者：陆广汇 

机构地区：[1]江苏省建湖县人民医院,224700

出　　处：《时珍国医国药》2004年第12期819-820,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的 :建立定量检测葛根素注射液中细菌内毒素的实验方法。方法 :采用《中国药典》2 0 0 0年版 ( 部 )附录检测细菌内毒素的动态浊度法。结果 :葛根素注射液用细菌内毒素定量法检测无干扰因素影响 ,内毒素回收率在 5 0 %～ 2 0 ) %范围内。结论 :使用动态浊度法定量检测葛根素注射液的细菌内毒素是可行的 。Objective:To develop an assay for de termination of bacterial endotoxin (BET) in Puerarin Injection.Methods:BET was determined by turbidimetric-kinetic meth od.Results:The content in Puerarin Injection did not interfer e with the quantitation of BET. The recovery of BET was between 50% and 200%.Conclusion:The method is proved to be suitable for determi nation of the BET in Puerarin Injection and can be used as an alternative for th e rabbit pyrogen test to the injection.

关 键 词：葛根素注射液 细菌内毒素 动态浊度法 干扰实验 

分 类 号：R284.2[医药卫生—中药学]