颗粒体病毒在小菜蛾种群中的垂直传播方式的初步观察  

Preliminary observation on vertical transmission of the granulosis virus in the population of the diamondback moth, Plutella xylostella

作　　者：吕利华[1] 何余容[2] 

机构地区：[1]广东省农业科学院植物保护研究所,广州510640 [2]华南农业大学资源环境学院昆虫生态室,广州510642

出　　处：《昆虫知识》2004年第6期559-562,共4页Entomological Knowledge

基　　金：国家自然科学基金项目资助 (39930 12 0 ;30 2 70 96 6 )

摘　　要：在室内观察了小菜蛾颗粒体病毒PlutellaxylostellaGranulosisVirus(RxGV)在小菜蛾种群水平上可能的垂直传播途径。结果表明 :(1 )上代小菜蛾Plutellaxylostella(L .)幼虫期饲毒不能将小菜蛾颗粒体病垂直传递到下代 ;(2 )取食病毒的雌蛾产的卵所孵化的幼虫中 ,没病毒感染出现 ,说明病毒不能经小菜蛾成虫体内传播到下一代。 (3 )用小菜蛾颗粒体病毒处理卵卡时 ,其初孵幼虫有少量被病毒感染死亡 ,浓度高时感染率也较高 ,但感染率均在 1 0 %以下 ,说明小菜蛾颗粒体病毒能经卵表垂直传递给下代 ,但传递效率不高。但当用病毒处理叶片上的卵时 ,初孵幼虫死亡率高达 77 73 % ,而且孵化出的幼虫大多数染病且死于 3龄以前 ,因此田间病毒的施用时间可提早到卵高峰期。The vertical transmission of Plutella xylostella granulosis virus (PxGV) was observed in the laboratory. No infected larvae were observed in the progeny of adults developed from virus-fed larvae. The granulosis virus of diamondback moth, when applied as a mixture of honey solution with virus to feed adults, was not transmitted to their progeny. Pollution of the diamondback moth eggs laid on a paper card with the virus suspension had no significant effect on hatching percentage of eggs between treated and untreated groups, but caused less than 10% mortality of hatched larvae before the third instar. However, contamination of the diamondback moth eggs laid on vegetable leaves with granulosis virus suspension could cause as high as 77.73% mortality of hatched larvae before the third instar. Therefore, the appropriate time for spraying granulosis virus to control the diamondback moth in the field could be ahead to the egg peak of the diamondback moth instead of the peak of the second instar larvae.

关 键 词：小菜蛾 颗粒体病毒 幼虫 孵化 施用时间 种群 虫体 垂直传播 观察 病毒感染 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]