转基因食品的快速PCR鉴定  

Quick Identification of Genetically Modified Food by PCR

作　　者：赵有玺[1] 饶志明[1] 王正祥[1] 沈微[1] 方慧英[1] 诸葛健[1] 

机构地区：[1]江南大学 工业生物技术教育部重点实验室,江苏无锡214036

出　　处：《无锡轻工大学学报（食品与生物技术）》2004年第6期6-8,12,共4页Journal of Wuxi University of Light Industry

基　　金：国家自然科学基金项目(30300027)资助课题

摘　　要：用十六烷基-二甲基-乙基-溴化铵(CTAB)法快速高效地从样品中提取了可供分析的基因组DNA,经PCR扩增,鉴定了含有35S启动子和NOS终止子的转基因样品.该方法的灵敏度可达0.1%,并且具有很好的稳定性.CTAB method was used to extract DNA from the food samples. This method is simple, fast and can make the consistency of DNA higher. It is suitable to extract large quantity DNA. PCR method is used for assaying transgenic component 35S promoter derived from Cauliflower Mosaic Virus and NOS terminator derived from Agrobacterium tumefaciens in foods. The sensitivity can reach 0.1% and the stability is excellent.

关 键 词：转基因食品检测 食品DNA提取 PCR 

分 类 号：TS207.7[轻工技术与工程—食品科学]