肝癌特异性AFP亚型测定方法及诊断价值研究  

Clinical value of hepatoma-specific alpha-fetoprotein in the diagnosis of primary hepatocellular carcinoma

作　　者：金飞[1] 倪润洲[1] 肖明兵[1] 黄介飞[1] 孟宪镛[1] 

机构地区：[1]南通大学附属医院消化内科,南通226001

出　　处：《南通医学院学报》2004年第4期379-380,382,共3页ACTA Academiae Medicinae Nantong

基　　金：江苏省社会发展科技项目 (BS2 0 0 45 2 8)

摘　　要：目的 :研究肝癌特异性甲胎蛋白 (AFP)亚型 (HS-AFP)对原发性肝细胞癌 (PHC)的诊断和鉴别诊断价值。方法 :建立聚丙烯酰胺凝胶电泳和 Western blot分离检测血清 HS-AFP的方法 ,对 82例 PHC和 95例良性肝病患者 HS-AFP进行分离检测 ,分析 HS-AFP与血清 AFP浓度、肿瘤大小及门静脉转移的关系。结果 :肝癌患者 HS-AFP阳性者为 74.4% ,明显高于良性肝病(肝硬化、慢性肝炎和急性肝炎的阳性率分别为 9.1%、7.3 %和 10 .0 % )。 HS-AFP与血清 AFP浓度和肿瘤大小有一定相关性 ,而与有无门静脉转移关系不大。结论 :HS-AFP可提高 AFP阴性 PHC和早期 PHC的诊断敏感性 ,尤其对良恶性肝病所致的 AFP升高有较高鉴别诊断价值。Objective:To study the clinical value of hepatoma-specific alpha-fetoprotein(HS-AFP) in the diagnosis and differential diagnosis of primary hepatocellular carcinoma(PHC).Methods:HS-AFP was separated and determined with polyacrylamide electrophoresis combined with Western blot in 82 cases with PHC and 95 cases with benign liver diseases.The correlations between positive rates of HS-AFP and serum AFP concentration,tumor size as well as portal vein metastasis were analyzed.Results:The positive rate of HS-AFP in PHC was 74.4%,which was significantly higher than that in benign liver diseases(9.1%,7.3% and 10.0% for liver cirrhosis,chronic hepatitis and acute hepatitis respectively).HS-AFP is correlated with serum AFP concentration and tumor size to some extent,but not with portal vein metastasis.Conclusions:The diagnostic sensitivity can be increased by HS-AFP in PHC cases with negative AFP or at early stage.And HS-AFP is especially useful in distinguishing high AFP due to PHC from benign liver diseases.

关 键 词：原发性肝细胞癌 肝细胞癌特异性甲胎蛋白区带 聚丙烯酰胺凝胶电泳 免疫印迹 

分 类 号：R735.7[医药卫生—肿瘤]