肠道淋巴通道在梗阻性黄疸内毒素血症发生中作用的实验研究  

The effect of intestinal lymphatic transport on the enterogenous endotoxemia with obstructive jaundice in rats

作　　者：毛勤生[1] 周新泽[1] 夏玲[1] 倪耀辉[1] 陈瑞新[1] 于秀[1] 陆丽萍[1] 

机构地区：[1]南通大学附属医院普外科,南通226001

出　　处：《南通医学院学报》2004年第4期393-394,共2页ACTA Academiae Medicinae Nantong

摘　　要：目的 :探讨肠道淋巴通道在梗阻性黄疸 (梗黄 )内毒素血症发生中的作用。方法 :Wistar大鼠 60只随机分为 3组 :假手术组、梗黄组和梗黄 +胸导管结扎组 ,于造模后第 15天抽下腔静脉血检测其谷丙转氨酶、总胆红素、内毒素、TNF -α和 IL -6水平 ,并行肺组织病理检查。结果 :造模后 15天 ,梗黄组大鼠肝功能严重受损 ,并发生较为明显的内毒素血症 ,表现为谷丙转氨酶、总胆红素和内毒素、TNF -α和 IL -6水平明显升高 ,肺组织病理损害严重 ;梗黄 +胸导管结扎组大鼠肝功能情况与梗黄组相似 ,但内毒素、TNF-α、IL -6水平却较梗黄组明显降低 ,肺组织病理损害亦明显减轻。结论 :肠道淋巴通道在梗黄肠原性内毒素易位中起有重要作用 ,阻断该通道对防治梗黄时肠原性内毒素血症及其所致的肺损伤可能具有重要意义。Objective:To investigate the effect of intestinal lymphatic transport on the enterogenous endotoxemia with obstructive jaundice.Methods:60 wistar rats were randomly divided into three groups:the shame-operative(SO) group,obstructive jaundice(OJ) group,and obstructive jaundice with thoracic duct ligation(OJTDL) group.On the 15th day postoperation,the vena cava inferior blood was obtained to determine the serum concentration of glutamicpyruvic transminase (GPT)、total bilirubin(Tbi)、endotoxin(ET)、TNF-α and IL-6.The lung was observed pathologically too.Results:On the 15th day postoperation,the levels of serum GPT、TBi、ET、TNF-α and IL-6 in OJ group were increased significantly as compared to those in SO group,and the pathologic damage of lung was severe.In OJTDL group,the levels of serum GPT and TBi were the same as those in OJ group,but the levels of serum ET、TNF-α and IL-6 were significantly lower than those in OJ group,and the serverity of lung injury was dramatically improved.Conclusion:Intestinal lymphatic transport has great effect on the translocation of enterogenous endotoxin with obstructive jaundice.Interrupting this transport may has great value in proventing the enterogenous endotoxemia and the lung injury with obstructive jaundice.

关 键 词：梗阻性黄疸 内毒素血症 胸导管 

分 类 号：R366[医药卫生—病理学]